Difference between revisions of "User:Samiye Yaman/Notebook/Lab 581/2013/03/29"

From OpenWetWare
< User:Samiye Yaman‎ | Notebook‎ | Lab 581‎ | 2013‎ | 03
Line 38: Line 38:

Revision as of 11:27, 12 April 2013

BDLlogo notext lr.png Experimental Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Part A: Preparing Dilutions for UV-Vis Measurements

  • Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times.
  • Prepare stock solutions for comprison (10μL, 15μL, 20μL, 25μL, 30μL and 35μL in 1.5 ml phosphate buffer)
  • UV-Vis measurements of stock solutions and supernatants were taken

Part B: Observing pH changes

  • Separate crystal violet containing hydrogels into two epi-test tubes, labelled as A & B.(app 1mg in each)
  • Add 1mL of 1M HCl to one epi test tube A and 1mL NaOH to epitest tube B.

3) Centrifuge for 2 min at 11200rpm.

4) Take UV-Vis of supernatants

Part C: Prepare hydrogels with further pH analysis

  • 0.5mg samples of PVOH hydrogels containing no lecithin and no dye was added in to 3 seperate epi test tubes with 1.5 mL phosphate buffer.


Graf 10ul.png

Graf 25ul.png