Difference between revisions of "User:Samiye Yaman/Notebook/Lab 581/2013/03/29"

From OpenWetWare
< User:Samiye Yaman‎ | Notebook‎ | Lab 581‎ | 2013‎ | 03
Jump to: navigation, search
(Description)
(fix raw html notebook nav)
 
(3 intermediate revisions by one other user not shown)
Line 2: Line 2:
 
|-
 
|-
 
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
 
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
Line 14: Line 14:
  
 
==Description==
 
==Description==
 +
 +
'''Part A: Preparing Dilutions for UV-Vis Measurements'''
 +
 +
*Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times.
 +
 +
*Prepare stock solutions for comprison (10μL, 15μL, 20μL, 25μL, 30μL and 35μL in 1.5 ml phosphate buffer)
 +
 +
* UV-Vis measurements of stock solutions and supernatants were taken
 +
 +
'''Part B: Observing pH changes'''
 +
 +
*Separate crystal violet containing hydrogels into two epi-test tubes, labelled as A & B.(app 1mg in each)
 +
 +
*Add 1mL of 1M HCl to one epi test tube A and 1mL NaOH to epitest tube B.
 +
 +
3) Centrifuge for 2 min at 11200rpm.
 +
 +
4) Take UV-Vis of supernatants
 +
 +
'''Part C: Prepare hydrogels with further pH analysis'''
 +
 +
*0.5mg samples of PVOH hydrogels containing no lecithin and no dye was added in to 3 seperate epi test tubes with 1.5 mL phosphate buffer.
  
 
==Data==
 
==Data==
 +
 +
[[Image:Graf_10ul.png]]
 +
 +
[[Image:15ul.png]]
 +
 +
[[Image:20ul.png]]
 +
 +
[[Image:Graf_25ul.png]]
 +
 +
[[Image:30ul.png]]
 +
 +
[[Image:35ul.png]]
  
 
==Notes==
 
==Notes==

Latest revision as of 21:35, 26 September 2017

BDLlogo notext lr.png Experimental Chemistry Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png



Objectives

Description

Part A: Preparing Dilutions for UV-Vis Measurements

  • Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times.
  • Prepare stock solutions for comprison (10μL, 15μL, 20μL, 25μL, 30μL and 35μL in 1.5 ml phosphate buffer)
  • UV-Vis measurements of stock solutions and supernatants were taken

Part B: Observing pH changes

  • Separate crystal violet containing hydrogels into two epi-test tubes, labelled as A & B.(app 1mg in each)
  • Add 1mL of 1M HCl to one epi test tube A and 1mL NaOH to epitest tube B.

3) Centrifuge for 2 min at 11200rpm.

4) Take UV-Vis of supernatants

Part C: Prepare hydrogels with further pH analysis

  • 0.5mg samples of PVOH hydrogels containing no lecithin and no dye was added in to 3 seperate epi test tubes with 1.5 mL phosphate buffer.

Data

Graf 10ul.png

15ul.png

20ul.png

Graf 25ul.png

30ul.png

35ul.png

Notes