Difference between revisions of "User:Samiye Yaman/Notebook/Lab 581/2013/03/27"

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(Description)
(Description)
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==Description==
 
==Description==
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'''Part 1: Freeze-thaw vortex cycle of liposomes'''
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 +
*5 freeze-thaw vortex cycle for liposome coated hydrogel. (vortex for 30sec, freeze (completely) by using liquid nitrogen then thaw (completely)in a water bath. Repeat the cycle 5 times.
 +
 
 +
*Centrifuge the solution(4000rpm for 5 min) then remove the 25mM Tris solution from the epi-test tube.
 +
 
 +
*Add 1.5 mL of 25mM Tris buffer back to the epi test tube with hydrogels.
 +
 
 +
 
 +
'''Part 2: Prepare a concentration range for R6G'''
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 +
*Add 2mg of PVOH to 6 different epi test tubes.
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*Add 1.5 ml of phosphate buffer.
 +
 
 +
*Add different volume of 1mM R6G to each epi test tube. (10μL, 15μL, 20μL, 25μL, 30μL, 35μL)
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==Data==
 
==Data==
  
 
==Notes==
 
==Notes==

Revision as of 07:18, 27 March 2013

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Objective

Description

Part 1: Freeze-thaw vortex cycle of liposomes

  • 5 freeze-thaw vortex cycle for liposome coated hydrogel. (vortex for 30sec, freeze (completely) by using liquid nitrogen then thaw (completely)in a water bath. Repeat the cycle 5 times.
  • Centrifuge the solution(4000rpm for 5 min) then remove the 25mM Tris solution from the epi-test tube.
  • Add 1.5 mL of 25mM Tris buffer back to the epi test tube with hydrogels.


Part 2: Prepare a concentration range for R6G

  • Add 2mg of PVOH to 6 different epi test tubes.
  • Add 1.5 ml of phosphate buffer.
  • Add different volume of 1mM R6G to each epi test tube. (10μL, 15μL, 20μL, 25μL, 30μL, 35μL)

Data

Notes