User:Samiye Yaman/Notebook/Lab 581/2013/03/27: Difference between revisions
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==Description== | ==Description== | ||
'''Part 1: Freeze-thaw vortex cycle of liposomes''' | |||
*5 freeze-thaw vortex cycle for liposome coated hydrogel. (vortex for 30sec, freeze (completely) by using liquid nitrogen then thaw (completely)in a water bath. Repeat the cycle 5 times. | |||
*Centrifuge the solution(4000rpm for 5 min) then remove the 25mM Tris solution from the epi-test tube. | |||
*Add 1.5 mL of 25mM Tris buffer back to the epi test tube with hydrogels. | |||
'''Part 2: Prepare a concentration range for R6G''' | |||
*Add 2mg of PVOH to 6 different epi test tubes. | |||
*Add 1.5 ml of phosphate buffer. | |||
*Add different volume of 1mM R6G to each epi test tube. (10μL, 15μL, 20μL, 25μL, 30μL, 35μL) | |||
==Data== | ==Data== | ||
==Notes== | ==Notes== | ||
Revision as of 08:18, 27 March 2013
 Experimental Chemistry
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ObjectiveDescriptionPart 1: Freeze-thaw vortex cycle of liposomes
DataNotes |
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