Difference between revisions of "User:Samiye Yaman/Notebook/Lab 581/2013/03/22"

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(Description)
(Description)
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*Add 1.5ml of water in to a epitest tube with 2mg hydrogel.
 
*Add 1.5ml of water in to a epitest tube with 2mg hydrogel.
  
*Centrifuge sample containing hydrogels and crystal violet indicator 3 times (5 minutes, 13200 rpm)
+
*Centrifuge sample containing hydrogels and crystal violet indicator 3 times for 5 minutes at 13200 rpm.
  
-Measure the absorbance of each supernatant to find the amount of CV that the hydrogels have absorbed
+
*By using UV-Vis, measure the absorbance of each supernatant to find the amount of crytal violet in the supernatent. Use the data to find out the amount of crystal violet absorbed by the hydrogels.(Initial amount of indicator added to the epi test tube is 20uL)
  
2) Prepare 2 more hydrogel solutions with the following indicators (these will be used to measure dye leakage as well as to monitor pH changes)
+
*Prepare 2 more hydrogel solutions (with 1.5 water and 2mg hydrogel) with 55 μL methyl red and 30μL bromophenol blue.
 
 
a) methyl red (55μL) in 1.5 mL ethanol w/ 2mg hydrogels
 
b) bromophenol blue (30μL) in 1.5 mL water w/ 2mg hydrogels
 
  
 
==Data==
 
==Data==
  
 
==Notes==
 
==Notes==

Revision as of 14:09, 6 April 2013

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Objective

Description

Part A:Preparing hydrogels for pH analysis

  • Add 1.5ml of water in to a epitest tube with 2mg hydrogel.
  • Centrifuge sample containing hydrogels and crystal violet indicator 3 times for 5 minutes at 13200 rpm.
  • By using UV-Vis, measure the absorbance of each supernatant to find the amount of crytal violet in the supernatent. Use the data to find out the amount of crystal violet absorbed by the hydrogels.(Initial amount of indicator added to the epi test tube is 20uL)
  • Prepare 2 more hydrogel solutions (with 1.5 water and 2mg hydrogel) with 55 μL methyl red and 30μL bromophenol blue.

Data

Notes