Difference between revisions of "User:Samiye Yaman/Notebook/Lab 581/2013/03/22"

From OpenWetWare
< User:Samiye Yaman‎ | Notebook‎ | Lab 581‎ | 2013‎ | 03
Jump to: navigation, search
(Description)
(Description)
Line 15: Line 15:
 
==Description==
 
==Description==
  
'''Part A'''
+
'''Part A:Preparing hydrogels for pH analysis'''
+
 
 +
*Add 1.5ml of water in to a epitest tube with 2mg hydrogel.
 +
 
 +
*Centrifuge sample containing hydrogels and crystal violet indicator 3 times (5 minutes, 13200 rpm)
 +
 
 +
-Measure the absorbance of each supernatant to find the amount of CV that the hydrogels have absorbed
 +
 
 +
2) Prepare 2 more hydrogel solutions with the following indicators (these will be used to measure dye leakage as well as to monitor pH changes)
 +
 
 +
a) methyl red (55μL) in 1.5 mL ethanol w/ 2mg hydrogels
 +
b) bromophenol blue (30μL) in 1.5 mL water w/ 2mg hydrogels
 +
 
 
==Data==
 
==Data==
  
 
==Notes==
 
==Notes==

Revision as of 07:48, 27 March 2013

BDLlogo notext lr.png Experimental Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Description

Part A:Preparing hydrogels for pH analysis

  • Add 1.5ml of water in to a epitest tube with 2mg hydrogel.
  • Centrifuge sample containing hydrogels and crystal violet indicator 3 times (5 minutes, 13200 rpm)

-Measure the absorbance of each supernatant to find the amount of CV that the hydrogels have absorbed

2) Prepare 2 more hydrogel solutions with the following indicators (these will be used to measure dye leakage as well as to monitor pH changes)

a) methyl red (55μL) in 1.5 mL ethanol w/ 2mg hydrogels b) bromophenol blue (30μL) in 1.5 mL water w/ 2mg hydrogels

Data

Notes