User:Samiye Yaman/Notebook/Lab 581/2013/03/08: Difference between revisions

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==Objectives==


==Description==
==Description==
*0.1 g of PVOH with 0.25g of lecithin(synthesized on http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/02/22)is placed in to a vial. 5 ml of phosphate buffer was added. The solution with 0.1 f of PVOH with lecithin was sonicated for half an hour. 5μl of of R6G was added. In 15 minutes intervals, UV-vis measurements was taken to measure the amount of dye left in the solution. 1 ml of distilled water was added to the solution every time 1 ml was taken out for UV-Vis measurement.
*The course is designed by American University to experiment different aspects of PVOH films.
 
*0.1 g of PVOH without lecithin(synthesized on http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/02/22)is placed in to a vial. 5 ml of phosphate buffer was added. The solution with 0.1 f of PVOH without lecithin was sonicated for half an hour. 5μl of of R6G was added. In 15 minutes intervals, UV-vis measurements was taken to measure the amount of dye left in the solution. 1 ml of distilled water was added to the solution every time 1 ml was taken out for UV-Vis measurement.
 
 
*2 mg of PVOH with R6G (without lecithin) was placed in 1.5ml of 5 different solution. These solutions were water, phosphate buffer, 1M of NaOH, 1M of HCl and fixant solution. Each PVOH stayed in solution for an hour before centrifuged. 5 minutes centrifuging is followed by UV-Vis measurements of the solutions.
 
==Data==
 
[[Image:Lecithin_leakage.png]]
 
shows the UV-Vis spectra of the 0.1 g of hydrogel in 5 ml of phosphate buffer (series 1) and 0.1 g of hydrogel with lecithin in 5ml of buffer (series 2). Absorbance of PVOH with lecithin was lower than the observance of PVOH without lecithin. It was indicated that use of lecithin decreases the absorbance of the PVOH.
 
 
[[Image:Dye_leakege_in_different_solutions.png]]


*During the course PVOH films with different molecular weight will be produced. Effects of cross-linking, addition of different molecules such as gluteraldehyde, different acids, porphrin will be also tested.
shows the UV-Vis spectra of nanohydrogels in different solutions. Series 1 corresponds to the results obtained from hydrogel being in water, series 2 in phosphate buffer, series 3 in 1 M NaOH,  series 4 in 1M of HCl and series 5 in fixant solution. During the synthesis of each hydrogel R6G dye was added. R6G dye should show a peak at 480nm in UV-Vis spectra. Each hydrogel with R6G dye attached stayed in the solution for 2 hours. The results indicate that there was no R6G dye leakage from the hydrogels to each solution as no peaks were observed around 480nm.


*UV-Vis spectrum, Atomic Absorption spectrum, X-Ray, FTIR, DMA, ISE and many other instruments will be used to analyze the results.
==Notes==

Revision as of 12:22, 22 March 2013

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Objectives

Description

  • 0.1 g of PVOH with 0.25g of lecithin(synthesized on http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/02/22)is placed in to a vial. 5 ml of phosphate buffer was added. The solution with 0.1 f of PVOH with lecithin was sonicated for half an hour. 5μl of of R6G was added. In 15 minutes intervals, UV-vis measurements was taken to measure the amount of dye left in the solution. 1 ml of distilled water was added to the solution every time 1 ml was taken out for UV-Vis measurement.
  • 0.1 g of PVOH without lecithin(synthesized on http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/02/22)is placed in to a vial. 5 ml of phosphate buffer was added. The solution with 0.1 f of PVOH without lecithin was sonicated for half an hour. 5μl of of R6G was added. In 15 minutes intervals, UV-vis measurements was taken to measure the amount of dye left in the solution. 1 ml of distilled water was added to the solution every time 1 ml was taken out for UV-Vis measurement.


  • 2 mg of PVOH with R6G (without lecithin) was placed in 1.5ml of 5 different solution. These solutions were water, phosphate buffer, 1M of NaOH, 1M of HCl and fixant solution. Each PVOH stayed in solution for an hour before centrifuged. 5 minutes centrifuging is followed by UV-Vis measurements of the solutions.

Data

shows the UV-Vis spectra of the 0.1 g of hydrogel in 5 ml of phosphate buffer (series 1) and 0.1 g of hydrogel with lecithin in 5ml of buffer (series 2). Absorbance of PVOH with lecithin was lower than the observance of PVOH without lecithin. It was indicated that use of lecithin decreases the absorbance of the PVOH.


shows the UV-Vis spectra of nanohydrogels in different solutions. Series 1 corresponds to the results obtained from hydrogel being in water, series 2 in phosphate buffer, series 3 in 1 M NaOH, series 4 in 1M of HCl and series 5 in fixant solution. During the synthesis of each hydrogel R6G dye was added. R6G dye should show a peak at 480nm in UV-Vis spectra. Each hydrogel with R6G dye attached stayed in the solution for 2 hours. The results indicate that there was no R6G dye leakage from the hydrogels to each solution as no peaks were observed around 480nm.

Notes
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