Difference between revisions of "User:Samiye Yaman/Notebook/Lab 581/2013/03/01"

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==Description==
 
==Description==
  
 +
'''Part 1'''
 
*Place a small piece of the hydrogels (synthesized on 22/02/2012) in a water solution.
 
*Place a small piece of the hydrogels (synthesized on 22/02/2012) in a water solution.
  
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*Sonicate each samples.
 
*Sonicate each samples.
 +
 +
*Fluorescence of samples with dyes already attached were measured by using fluorometer.
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 +
'''Part 2'''
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*0.3 g of sample with and without lecithin placed in to 2 different vials.
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 +
*Add 6ml of water and sonicate the samples.
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 +
*Take 1 ml of each sample add in to 1.5ml microcentrifuge tubes. Centrifuge samples.( Additionally pipette it up and down to break the larger pieces.)
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*Add 1μl of R6G to each microcentrifuge tubes to be absorbed by the PVOH.
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 +
*Fluorescence of samples with dyes already attached were measured by using fluorometer.
  
 
==Data==
 
==Data==
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[[Image:0.5gpva_0.25gl_lecithin_in_safflower_oil.png]]
  
 
==Notes==
 
==Notes==

Revision as of 20:19, 19 March 2013

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Objections

Description

Part 1

  • Place a small piece of the hydrogels (synthesized on 22/02/2012) in a water solution.
  • Observe under the microscope.
  • Sonicate each samples.
  • Fluorescence of samples with dyes already attached were measured by using fluorometer.

Part 2

  • 0.3 g of sample with and without lecithin placed in to 2 different vials.
  • Add 6ml of water and sonicate the samples.
  • Take 1 ml of each sample add in to 1.5ml microcentrifuge tubes. Centrifuge samples.( Additionally pipette it up and down to break the larger pieces.)
  • Add 1μl of R6G to each microcentrifuge tubes to be absorbed by the PVOH.
  • Fluorescence of samples with dyes already attached were measured by using fluorometer.

Data

0.5gpva 0.25gl lecithin in safflower oil.png

Notes