User:Samiye Yaman/Notebook/Lab 581/2013/02/22

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Objections

Description

Part 1

  • Prepare 3 sets of hydrogels with phosphate buffers

A)0.5 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.

B)0.5 g PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer and 0.25g lecithin was placed on to a heat block at 100C.

C)0.5 g PVOH (MW:89,000-98,000) with 5 ml of phosphate buffer, 10μL R6G, and 0.25g lecithin was placed on to a heat block at 100C.

D) 0.5 g PVOH (MW:89,000-98,000) with 5 ml of phosphate buffer and 10μL R6G was placed on to a heat block at 100C.

  • Cool down the solutions before homoginizing with safflower oil.
  • Homoginize each of the hydrogels solutions with 50 ml of safflower oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle, 20 hours freeze 4 hours room temperature)

Data

Notes