User:Samiye Yaman/Notebook/Lab 581/2013/02/22: Difference between revisions
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==Description== | ==Description== | ||
'''Part 1''' | |||
*Prepare 3 sets of hydrogels with phosphate buffers | |||
A)0.5 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. | |||
B)0.5 g PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer and 0.25g lecithin was placed on to a heat block at 100C. | |||
C)0.5 g PVOH (MW:89,000-98,000) with 5 ml of phosphate buffer, 10μL R6G, and 0.25g lecithin was placed on to a heat block at 100C. | |||
D) 0.5 g PVOH (MW:89,000-98,000) with 5 ml of phosphate buffer and 10μL R6G was placed on to a heat block at 100C. | |||
*Cool down the solutions before homoginizing with safflower oil. | |||
*Homoginize each of the hydrogels solutions with 50 ml of safflower oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle, 20 hours freeze 4 hours room temperature) | |||
==Data== | ==Data== | ||
==Notes== | ==Notes== | ||
Revision as of 10:38, 1 March 2013
 Experimental Chemistry
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ObjectionsDescriptionPart 1
A)0.5 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. B)0.5 g PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer and 0.25g lecithin was placed on to a heat block at 100C. C)0.5 g PVOH (MW:89,000-98,000) with 5 ml of phosphate buffer, 10μL R6G, and 0.25g lecithin was placed on to a heat block at 100C. D) 0.5 g PVOH (MW:89,000-98,000) with 5 ml of phosphate buffer and 10μL R6G was placed on to a heat block at 100C.
DataNotes |
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