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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
 
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.
 
C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.
  
*Add 2μ of R6G were added for each ml of phophate buffer. Add 6μ R6G in to B and C. Add 8μ to A.
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*Homoginize each of the hydrogels solutions with 35 ml of safflower oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle, 20 hours freeze 4 hours room temperature)
 
 
*Homoginize each of the hydrogels solutions with 35 ml of safflower oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle)
 
  
  
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==Notes==
 
==Notes==
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*Safflower oil was used instead of mineral oil. Safflower oil has lower freezing point (approximately -17) than mineral oil (fp:-30C)
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The reason why we wanted to try safflower oil was it has freezing point lower than -20C. Mineral oil did not freeze completely at -20C.
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*We used big petri dishes instead of beakers to increase the surface area and have a less dense medium as hydrogels sink at the bottom of the container. (to observe if larger surface area will make any difference)
 +
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*Liquid nitrogen was used to flash freeze the hydrogels before they sink at the bottom of the container.

Latest revision as of 21:28, 26 September 2017

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Objections

Description

Part 1

  • Prepare 3 sets of hydrogels with phosphate buffers

A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C.

B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.

C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.

  • Homoginize each of the hydrogels solutions with 35 ml of safflower oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle, 20 hours freeze 4 hours room temperature)


Part 2

  • Prepare 3 sets of hydrogels with phosphate buffers

A)0.52 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C.

B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.

C)0.13 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.

  • Homoginize each of the hydrogels solutions with 50 ml of safflower oil. Pour them in to a petri dish to have a larger surface area then place them into freezer.(-20C) (3 freeze thaw cycle)


Part 3

  • Prepare a set of hydrogel with phosphate buffer

A)0.25 g POVH (MW:89,000-98,000) with 4 ml of phosphate buffer was blaced to a heat block at 100C.

  • Homoginize each of the hydrogels solutions with 50 ml of mineral oil. Pour them in to a petri dish to have a larger surface area then place it into the freezer.(-20C) (3 freeze thaw cycle)

Data

Notes

  • Safflower oil was used instead of mineral oil. Safflower oil has lower freezing point (approximately -17) than mineral oil (fp:-30C)

The reason why we wanted to try safflower oil was it has freezing point lower than -20C. Mineral oil did not freeze completely at -20C.

  • We used big petri dishes instead of beakers to increase the surface area and have a less dense medium as hydrogels sink at the bottom of the container. (to observe if larger surface area will make any difference)
  • Liquid nitrogen was used to flash freeze the hydrogels before they sink at the bottom of the container.