Difference between revisions of "User:Samiye Yaman/Notebook/Lab 581/2013/02/20"

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===Objections==
+
==Objections==
  
 
==Description==
 
==Description==
  
*Prepare 3 sets of hydrogels with phosphate buffers
+
'''Part 1'''
 +
 
 +
*Prepare 3 sets of hydrogels with phosphate buffers
  
 
A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C.
 
A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C.
Line 23: Line 25:
 
C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.
 
C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.
  
*Add 2μ of R6G were added for each ml of phophate buffer. Add 6μ R6G in to B and C. Add 8μ to A.
+
*Homoginize each of the hydrogels solutions with 35 ml of safflower oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle, 20 hours freeze 4 hours room temperature)
 +
 
 +
 
 +
'''Part 2'''
 +
 
 +
*Prepare 3 sets of hydrogels with phosphate buffers
 +
 
 +
A)0.52 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C.
 +
 
 +
B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.
 +
 
 +
C)0.13 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.
  
*Homoginize each of the hydrogels solutions with 50 ml of mineral oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle)
+
*Homoginize each of the hydrogels solutions with 50 ml of safflower oil. Pour them in to a petri dish to have a larger surface area then place them into freezer.(-20C) (3 freeze thaw cycle)
  
 +
 +
'''Part 3'''
 +
 +
*Prepare a set of hydrogel with phosphate buffer
 +
 +
A)0.25 g POVH (MW:89,000-98,000) with 4 ml of phosphate buffer was blaced to a heat block at 100C.
 +
 +
*Homoginize each of the hydrogels solutions with 50 ml of mineral oil. Pour them in to a petri dish to have a larger surface area then place it into the freezer.(-20C) (3 freeze thaw cycle)
  
 
==Data==
 
==Data==
  
 
==Notes==
 
==Notes==
 +
 +
*Safflower oil was used instead of mineral oil. Safflower oil has lower freezing point (approximately -17) than mineral oil (fp:-30C)
 +
The reason why we wanted to try safflower oil was it has freezing point lower than -20C. Mineral oil did not freeze completely at -20C.
 +
 +
*We used big petri dishes instead of beakers to increase the surface area and have a less dense medium as hydrogels sink at the bottom of the container. (to observe if larger surface area will make any difference)
 +
 +
*Liquid nitrogen was used to flash freeze the hydrogels before they sink at the bottom of the container.

Revision as of 08:39, 20 February 2013

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Objections

Description

Part 1

  • Prepare 3 sets of hydrogels with phosphate buffers

A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C.

B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.

C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.

  • Homoginize each of the hydrogels solutions with 35 ml of safflower oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle, 20 hours freeze 4 hours room temperature)


Part 2

  • Prepare 3 sets of hydrogels with phosphate buffers

A)0.52 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C.

B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.

C)0.13 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.

  • Homoginize each of the hydrogels solutions with 50 ml of safflower oil. Pour them in to a petri dish to have a larger surface area then place them into freezer.(-20C) (3 freeze thaw cycle)


Part 3

  • Prepare a set of hydrogel with phosphate buffer

A)0.25 g POVH (MW:89,000-98,000) with 4 ml of phosphate buffer was blaced to a heat block at 100C.

  • Homoginize each of the hydrogels solutions with 50 ml of mineral oil. Pour them in to a petri dish to have a larger surface area then place it into the freezer.(-20C) (3 freeze thaw cycle)

Data

Notes

  • Safflower oil was used instead of mineral oil. Safflower oil has lower freezing point (approximately -17) than mineral oil (fp:-30C)

The reason why we wanted to try safflower oil was it has freezing point lower than -20C. Mineral oil did not freeze completely at -20C.

  • We used big petri dishes instead of beakers to increase the surface area and have a less dense medium as hydrogels sink at the bottom of the container. (to observe if larger surface area will make any difference)
  • Liquid nitrogen was used to flash freeze the hydrogels before they sink at the bottom of the container.