User:Samiye Yaman/Notebook/Lab 581/2013/02/06

From OpenWetWare
< User:Samiye Yaman‎ | Notebook‎ | Lab 581‎ | 2013‎ | 02
Revision as of 08:25, 6 February 2013 by Samiye Yaman (talk | contribs) (Description)
Jump to: navigation, search
BDLlogo notext lr.png Experimental Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Description

  • Hydrogels synthesized on 01/02/2013 was transferred from -20C to room temperature.

Part A Synthesizing Phosphate Buffer

  • Concentration Needed; 20mMol
  • pH Needed: 7.4
  • pka: 7.3m
  • base: sodium monophosphate
  • acid: sodium diphosphate
  • Acid + Base= 20 mmol therefore Base= 20mmol-acid
  • pH= pka+ log (base/acid)
  • (20mmol-acid)/acid= pH/pka

(Base/acid)=1.25.

  • Acid=8.85mmol & Base= 11.15mmol

Mass used for base: (119.98 g/mol)* 0.01115mol= 1.338g

Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g

  • Add 1.25 g of sodium diphosphate and 1.34g of sodium monophosphate in to a 900ml of miliQ water. (Stir well to make sure it is completely homogeneous). Ph needs to be 7.4 so 0.5M of sodium hydroxide was added drop by drop to make sure Ph reaches to 7.4.

Part B Dissolving PVOH with phosphate buffer

  • 0.51 g of PVOH (MW:89000-98000) was placed in to a beaker with 5 ml of water. It was placed in to a hot plate with temperature of 120C.(stir well to dissolve PVOH completely).Add 2µ of R6G.

Data

Notes