User:Samiye Yaman/Notebook/Lab 581/2013/02/06

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  • Hydrogels synthesized on 01/02/2013 was transferred from -20C to room temperature.

Part A Synthesizing Phosphate Buffer

  • Concentration Needed; 20mMol
  • pH Needed: 7.4
  • pka: 7.3m
  • base: sodium monophosphate
  • acid: sodium diphosphate
  • Acid + Base= 20 mmol therefore Base= 20mmol-acid
  • pH= pka+ log (base/acid)
  • (20mmol-acid)/acid= pH/pka


  • Acid=8.85mmol & Base= 11.15mmol

Mass used for base: (119.98 g/mol)* 0.01115mol= 1.338g

Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g

  • Add 1.25 g of sodium diphosphate and 1.34g of sodium monophosphate in to a 900ml of miliQ water. (Stir well to make sure it is completely homogeneous). Ph needs to be 7.4 so 0.5M of sodium hydroxide was added drop by drop to make sure Ph reaches to 7.4.