User:Samiye Yaman/Notebook/Lab 581/2012/10/31: Difference between revisions

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==Objective==


==Description==
==Description==
*The course is designed by American University to experiment different aspects of PVOH films.


*During the course PVOH films with different molecular weight will be produced. Effects of cross-linking, addition of different molecules such as gluteraldehyde, different acids, porphrin will be also tested.
'''Part 1 : Preparation of porphyrin films'''
 
* Add 2 drops of HCL, 1 ml of gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down.
 
*Keep everything under the hood.
 
'''Part 2 : UV-VIS of porphyrin dilutions prepared on 17/10/2012'''
 
*'''A)''' from the UV-Vis results we concluded that it is necessary to make extra 2 dilutions as dilution 1 and 2 are out of range. They are too concentrated. We would like to obtain a peak lower than 1.
 
*Dilution 6: 4 ml of dilution 5 + 4 ml of HPLC water.(0.3375micM)
 
*Dilution 7: 4ml of dilution 6 + 4 ml of HPLC water. (0.16785micM)
 
'''Part 3 : Prepare dilutions of 50micM Na2SO4, 50micM Al2(SO4)3, 50micM Fe2(SO4)3''' (HPLC WATER IS USED)
 
'''Na2SO4'''
 
*FW: 142.07 g/ml
 
*1/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10 ml of HPLC water.
 
*(50mM) (x) = (50micM) (10mL)
         
* x  = 0.01 ml =10micL of Na2SO4 in 9.99ml HPLC water
 
 
'''Al2(SO4)3'''
 
*FW: 456.33g/mol
 
*456.33g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.392g in 10 mL H2O.
 
*50micM=10micL of 50mM in 999micL H2O
 
 
'''Fe2(SO4)3''' (72wt%)
 
*FW:399.88g/mol
 
* 0.72 x 399.88g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.144g in 10 mL H2O.
 
*50micM=10micL of 50mM in 999micL H2O
 
==Data==
 
'''ISE results for sodium sulfate dilutions'''
 
*Dilution 1(1.25ppm)= -180mV
 
*Dilution 2(2.5ppm)=  -182.6mV
 
*Dilution 3(5ppm)  = -182.4mV
 
*Dilution 4(10ppm )= -177.4mV
 
*Dilution 5(20ppm)= -174.6mV
 
 
*We decided detection limit was between dilution 1 and 2.
 
*More negative number means less sodium ion in the solution.
 
*The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.
 
==Notes==
This area is for any observations or conclusions that you would like to note.
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
[[Category:Course]]
[[Category:Miscellaneous]]
 
 
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}


*UV-Vis spectrum, Atomic Absorption spectrum, X-Ray, FTIR, DMA, ISE and many other instruments will be used to analyze the results.
__NOTOC__

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Objective

Description

Part 1 : Preparation of porphyrin films

  • Add 2 drops of HCL, 1 ml of gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down.
  • Keep everything under the hood.

Part 2 : UV-VIS of porphyrin dilutions prepared on 17/10/2012

  • A) from the UV-Vis results we concluded that it is necessary to make extra 2 dilutions as dilution 1 and 2 are out of range. They are too concentrated. We would like to obtain a peak lower than 1.
  • Dilution 6: 4 ml of dilution 5 + 4 ml of HPLC water.(0.3375micM)
  • Dilution 7: 4ml of dilution 6 + 4 ml of HPLC water. (0.16785micM)

Part 3 : Prepare dilutions of 50micM Na2SO4, 50micM Al2(SO4)3, 50micM Fe2(SO4)3 (HPLC WATER IS USED)

Na2SO4

  • FW: 142.07 g/ml
  • 1/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10 ml of HPLC water.
  • (50mM) (x) = (50micM) (10mL)
  • x = 0.01 ml =10micL of Na2SO4 in 9.99ml HPLC water


Al2(SO4)3

  • FW: 456.33g/mol
  • 456.33g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.392g in 10 mL H2O.
  • 50micM=10micL of 50mM in 999micL H2O


Fe2(SO4)3 (72wt%)

  • FW:399.88g/mol
  • 0.72 x 399.88g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.144g in 10 mL H2O.
  • 50micM=10micL of 50mM in 999micL H2O

Data

ISE results for sodium sulfate dilutions

  • Dilution 1(1.25ppm)= -180mV
  • Dilution 2(2.5ppm)= -182.6mV
  • Dilution 3(5ppm) = -182.4mV
  • Dilution 4(10ppm )= -177.4mV
  • Dilution 5(20ppm)= -174.6mV


  • We decided detection limit was between dilution 1 and 2.
  • More negative number means less sodium ion in the solution.
  • The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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