Difference between revisions of "User:Samiye Yaman/Notebook/Lab 581/2012/10/31"

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==Description==
 
==Description==
  
'''Part 1 : Preparation of porphyrin films'''
+
'''Part 1 '''
  
* Add 2 drops of HCL, 1 ml of gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down.
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*Porphyrin film which was cross-linked on 19/10/2012 was placed in to a 1% HCL solution with a volume of 100mL.  
  
*Keep everything under the hood.
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'''Part 2: Prepare a porphyrin solution of 50micM'''
  
'''Part 2 : UV-VIS of porphyrin dilutions prepared on 17/10/2012'''
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'''Part 3: UV-Vis'''
  
*'''A)''' from the UV-Vis results we concluded that it is necessary to make extra 2 dilutions as dilution 1 and 2 are out of range. They are too concentrated. We would like to obtain a peak lower than 1.
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*Combination of 1 ml of salt/acid (50micM), 1 ml of 50 micM of porphyrin and 2 ml of HPLC water.
 
 
*Dilution 6: 4 ml of dilution 5 + 4 ml of HPLC water.(0.3375micM)
 
 
 
*Dilution 7: 4ml of dilution 6 + 4 ml of HPLC water. (0.16785micM)
 
 
 
'''Part 3 : Prepare dilutions of 50micM Na2SO4, 50micM Al2(SO4)3, 50micM Fe2(SO4)3''' (HPLC WATER IS USED)
 
 
 
'''Na2SO4'''
 
 
 
*FW: 142.07 g/ml
 
 
 
*1/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10 ml of HPLC water.
 
 
 
*(50mM) (x) = (50micM) (10mL)
 
         
 
* x  = 0.01 ml =10micL of Na2SO4 in 9.99ml HPLC water
 
 
 
 
 
'''Al2(SO4)3'''
 
 
 
*FW: 456.33g/mol
 
 
 
*456.33g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.392g in 10 mL H2O.
 
 
 
*50micM=10micL of 50mM in 999micL H2O
 
 
 
 
 
'''Fe2(SO4)3''' (72wt%)
 
 
 
*FW:399.88g/mol
 
 
 
* 0.72 x 399.88g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.144g in 10 mL H2O.
 
 
 
*50micM=10micL of 50mM in 999micL H2O
 
  
 
==Data==
 
==Data==
  
'''ISE results for sodium sulfate dilutions'''
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'''Before placing porphyrin film in to 1 % HCl solution'''
 
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[[Image:2012-10-31_11.19.50.jpg|500px]]
*Dilution 1(1.25ppm)= -180mV
 
 
 
*Dilution 2(2.5ppm)=  -182.6mV
 
  
*Dilution 3(5ppm)  = -182.4mV
 
  
*Dilution 4(10ppm )= -177.4mV
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'''Right after placing porphyrin film into 1 % HCl solution'''
  
*Dilution 5(20ppm)= -174.6mV
+
*Edges of the film become more green.
  
 +
[[Image:2012-10-31_11.21.49.jpg|500px]]
  
*We decided detection limit was between dilution 1 and 2.
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'''48 hours after placing porphyrin film, prepared into 1 % HCl solution'''
  
*More negative number means less sodium ion in the solution.
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*The film became full green.(compared to the pictures on 31/10/2012)
  
*The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.
+
[[Image:2012-11-02_12.14.36.jpg|500px]]
  
 
==Notes==
 
==Notes==

Revision as of 11:28, 2 November 2012

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Objective

Description

Part 1

  • Porphyrin film which was cross-linked on 19/10/2012 was placed in to a 1% HCL solution with a volume of 100mL.

Part 2: Prepare a porphyrin solution of 50micM

Part 3: UV-Vis

  • Combination of 1 ml of salt/acid (50micM), 1 ml of 50 micM of porphyrin and 2 ml of HPLC water.

Data

Before placing porphyrin film in to 1 % HCl solution 2012-10-31 11.19.50.jpg


Right after placing porphyrin film into 1 % HCl solution

  • Edges of the film become more green.

2012-10-31 11.21.49.jpg

48 hours after placing porphyrin film, prepared into 1 % HCl solution

  • The film became full green.(compared to the pictures on 31/10/2012)

2012-11-02 12.14.36.jpg

Notes

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