|
Objective
Description
Part 1 : Preparation of porphyrin films
- Add 2 drops of HCL, 1 ml of gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down.
- Keep everything under the hood.
Part 2 : UV-VIS of porphyrin dilutions prepared on 17/10/2012
- A) from the UV-Vis results we concluded that it is necessary to make extra 2 dilutions as dilution 1 and 2 are out of range. They are too concentrated. We would like to obtain a peak lower than 1.
- Dilution 6: 4 ml of dilution 5 + 4 ml of HPLC water.(0.3375micM)
- Dilution 7: 4ml of dilution 6 + 4 ml of HPLC water. (0.16785micM)
Part 3 : Prepare dilutions of 50micM Na2SO4, 50micM Al2(SO4)3, 50micM Fe2(SO4)3 (HPLC WATER IS USED)
Na2SO4
- 1/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10 ml of HPLC water.
- (50mM) (x) = (50micM) (10mL)
- x = 0.01 ml =10micL of Na2SO4 in 9.99ml HPLC water
Al2(SO4)3
- 456.33g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.392g in 10 mL H2O.
- 50micM=10micL of 50mM in 999micL H2O
Fe2(SO4)3 (72wt%)
- 0.72 x 399.88g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.144g in 10 mL H2O.
- 50micM=10micL of 50mM in 999micL H2O
Data
ISE results for sodium sulfate dilutions
- Dilution 1(1.25ppm)= -180mV
- Dilution 2(2.5ppm)= -182.6mV
- Dilution 3(5ppm) = -182.4mV
- Dilution 4(10ppm )= -177.4mV
- Dilution 5(20ppm)= -174.6mV
- We decided detection limit was between dilution 1 and 2.
- More negative number means less sodium ion in the solution.
- The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.
Notes
This area is for any observations or conclusions that you would like to note.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
| 
Experimental Chemistry
