User:Samiye Yaman/Notebook/Lab 581/2012/10/24

From OpenWetWare
< User:Samiye Yaman‎ | Notebook‎ | Lab 581‎ | 2012‎ | 10
Revision as of 07:09, 31 October 2012 by Samiye Yaman (talk | contribs) (Description)
Jump to: navigation, search
BDLlogo notext lr.png Experimental Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Part 1 : Preparation of porphyrin films

  • Add 2 drops of HCL, 1 ml of gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down.
  • Keep everything under the hood.

Part 2 : UV-VIS of porphyrin dilutions prepared on 17/10/2012

  • A) from the UV-Vis results we concluded that it is necessary to make extra 2 dilutions as dilution 1 and 2 are out of range. They are too concentrated. We would like to obtain a peak lower than 1.
  • Dilution 6: 4 ml of dilution 5 + 4 ml of HPLC water.(0.3375micM)
  • Dilution 7: 4ml of dilution 6 + 4 ml of HPLC water. (0.16785micM)

Part 3 : Prepare dilutions of 50micM Na2SO4, 50micM Al2(SO4)3, 50micM Fe2(SO4)3 (HPLC WATER IS USED)


  • FW: 142.07 g/ml
  • 1/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10 ml of HPLC water.
  • (50mM) (x) = (50micM) (10mL)
  • x = 0.01 ml =10micL of Na2SO4 in 9.99ml HPLC water


  • FW: 456.33g/mol
  • 456.33g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.392g in 10 mL H2O.
  • 50micM=10micL of 50mM in 999micL H2O

Fe2(SO4)3 (72wt%)

  • FW:399.88g/mol
  • 0.72 x 399.88g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.144g in 10 mL H2O.
  • 50micM=10micL of 50mM in 999micL H2O


ISE results for sodium sulfate dilutions

  • Dilution 1(1.25ppm)= -180mV
  • Dilution 2(2.5ppm)= -182.6mV
  • Dilution 3(5ppm) = -182.4mV
  • Dilution 4(10ppm )= -177.4mV
  • Dilution 5(20ppm)= -174.6mV

  • We decided detection limit was between dilution 1 and 2.
  • More negative number means less sodium ion in the solution.
  • The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.


This area is for any observations or conclusions that you would like to note.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.