User:S Sibert/Notebook/SBB13Ntbk-Stephanie J Sibert

From OpenWetWare
< User:S Sibert‎ | Notebook
Revision as of 14:19, 1 December 2013 by S Sibert (talk | contribs) (4/2/13)
Jump to: navigation, search

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:S Sibert/Notebook/SBB13Ntbk-Stephanie J Sibert</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

Owwnotebook icon.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>











4/4/13

Ligations 6.5uL ddH2O 1uL Ligation buffer 1uL vector 1uL insert 0.5uL enzyme (last)

Comp Cells 1 tube cells 30uL KCM (no ddH2O)

4/9/13

no colonies in new plate, 3 colonies in old plate will be used to proceed.

picked colonies. by ejecting pipet tip into tube.

4/11/13

1)miniprep culture
2) restriction map EcoRI/XhoI
6ul H2O
1ul buffer
2ul DNA
0.5ul XhoI
0.5ul EcoRI
put in thermo cycler 11:23
3) run a gel
Lane 7-Sample A
Lane 8-Sample B
Lane 9-Sample C

4) No results.

4/16/13

Starting with
50ul rxn
31.5 ul H20
5ul dNTP
10ul Phusion buffer
1ul template (sample from 3/12/13)
1ul 10mm oligo 1 (CCOMT2-3 diluted)
1ul 10mm oligo 2 (CCOMT2-4 diluted)
0.5ul Phusion


Tube placed in thermocycler labeled ccomt2 4/16/13


Golden Gate Set up:
(n)ul ddH2O to 10ul
1ul Ligase Buffer
0.5ul Ligase
0.5ul BsmB1
1ul PER CONSTRUCT
0.5ul vertor

4/18/2013

sample processed by jc anderson to miniprep step
miniprep

4/19/2012

sequence confirmed
golden gate
Protocol:
6.5ul ddH2o
1ul ligase buffer
0.5ul ligase
0.5ul BsMb1
1ul of synthon mic
o.5ul vector

4/23/13

Transform
1 tube
20ul KCM
no h20
Rescue
plate

4/25/13

~miniprep~
6.5ul H20
2ul Plasmid
1ul NEB2
0.5ul Bsa1

~map~
lanes 7,8,9
A,B,D respectivly



~seq~

Recent changes

24 September 2017

N    22:33  MediaWiki:Tagline‎ (diff | hist) . . (+16). . Yardena Cohen (talk | contribs) (less parsing)
N    22:32  MediaWiki:Pagetitle-view-mainpage‎ (diff | hist) . . (+11). . Yardena Cohen (talk | contribs) (less parsing)
N    22:32  MediaWiki:Opensearch-desc‎ (diff | hist) . . (+16). . Yardena Cohen (talk | contribs) (english-only?)
N    21:21  MediaWiki:Pagetitle‎ (diff | hist) . . (+16). . Yardena Cohen (talk | contribs) (idk if this will help or hurt)
N    21:18  MediaWiki:Aboutsite‎ (diff | hist) . . (+17). . Yardena Cohen (talk | contribs) (one less thing to parse)

     20:08 

(Upload log). .

[Anna Hoge‎ (2×)]

     

20:08

. . Anna Hoge (talk | contribs) uploaded File:AHgraph4.png

     

18:52

. . Anna Hoge (talk | contribs) uploaded File:AHgraph3.png

23 September 2017

     17:17  MediaWiki:Contactpage-pagetext‎ (diff | hist) . . (+36). . Yardena Cohen (talk | contribs) (don't ask me science questions plz)

     17:05 

(Deletion log). .

[Yardena Cohen‎ (6×)]

     

17:05

. . Yardena Cohen (talk | contribs) deleted page User talk:Deepak P Patil(Automatically deleted when merging users)

     

17:05

. . Yardena Cohen (talk | contribs) deleted page User:Deepak P Patil(Automatically deleted when merging users)

     

16:39

. . Yardena Cohen (talk | contribs) deleted page User talk:Chun H Hsieh(Automatically deleted when merging users)

     

16:39

. . Yardena Cohen (talk | contribs) deleted page User:Chun H Hsieh(Automatically deleted when merging users)

     

15:39

. . Yardena Cohen (talk | contribs) deleted page User talk:Satu Strandman(Automatically deleted when merging users)

     

15:39

. . Yardena Cohen (talk | contribs) deleted page User:Satu Strandman(Automatically deleted when merging users)

     17:05 

(User merge log). .

[Yardena Cohen‎ (6×)]

     

17:05

. . Yardena Cohen (talk | contribs) Deleted user: Deepak P Patil (8544) ‎

     

17:05

. . Yardena Cohen (talk | contribs) User Deepak P Patil (8544) merged to Anonymous (0) ‎

     

16:39

. . Yardena Cohen (talk | contribs) Deleted user: Chun H Hsieh (13046) ‎

     

16:39

. . Yardena Cohen (talk | contribs) User Chun H Hsieh (13046) merged to Anonymous (0) ‎

     

15:39

. . Yardena Cohen (talk | contribs) Deleted user: Satu Strandman (13640) ‎

     

15:39

. . Yardena Cohen (talk | contribs) User Satu Strandman (13640) merged to Anonymous (0) ‎

N    14:04 

MediaWiki:Contactpage-label‎‎ (2 changes | history) . . (+7). . [Yardena Cohen‎ (2×)]

     

14:04

(cur | prev) . . (-4). . Yardena Cohen (talk | contribs) (one word so it doesn't get wrapped)

N    

13:59

(cur | prev) . . (+11). . Yardena Cohen (talk | contribs) (label text for footer?)

     14:02 

New server‎‎ (2 changes | history) . . (-137). . [Yardena Cohen‎ (2×)]

     

14:02

(cur | prev) . . (+10). . Yardena Cohen (talk | contribs) (contact form)

     

11:34

(cur | prev) . . (-147). . Yardena Cohen (talk | contribs) (it happened)

N    14:01 

MediaWiki:Contactpage-url‎‎ (4 changes | history) . . (+21). . [Yardena Cohen‎ (4×)]

     

14:01

(cur | prev) . . (-23). . Yardena Cohen (talk | contribs) (relative again)

     

14:01

(cur | prev) . . (-10). . Yardena Cohen (talk | contribs) (actual url)

     

13:59

(cur | prev) . . (+23). . Yardena Cohen (talk | contribs) (full url?)

N    

13:57

(cur | prev) . . (+31). . Yardena Cohen (talk | contribs) (relative link)

     13:52 

(User rights log). .

[Yardena Cohen‎ (2×)]

     

13:52

. . Yardena Cohen (talk | contribs) changed group membership for Oww Op from bureaucrat and administrator to staff ‎

     

13:52

. . Yardena Cohen (talk | contribs) changed group membership for Oww Op from (none) to administrator and bureaucrat ‎

     13:47 (User creation log) . . User account Oww Op (talk | contribs) was created by Yardena Cohen (talk | contribs) and password was sent by email ‎
     11:34 (Patrol log) . . Yardena Cohen (talk | contribs) automatically marked revision 996201 of page New server patrolled ‎
     11:33  BIOL398-04/S15:Class Assignments by Week‎ (diff | hist) . . (-3). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from Tessa A. Morris Class Assignments by Week to Tessa Morris Class Assignments by Week in a maintenance job.)
     11:33  Paris Bettencourt 2012/Notebook/modularity group/day by day/2012/07‎ (diff | hist) . . (+1). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from IGEM:Paris Bettencourt 2012/Notebook/modularity group/day by day//2012/07 to IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07 in a maintenance job.)
     11:33  Paris Bettencourt 2012/Notebook/modularity group/day by day/2012/07/03‎ (diff | hist) . . (+1). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from IGEM:Paris Bettencourt 2012/Notebook/modularity group/day by day//2012/07/03 to IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/03 in a maintenance job.)
     11:33  BIOMOD/2012/TeamSendaiA/Methods‎ (diff | hist) . . (-1). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from BIOMOD/2012/TeamSendaiA/Methodsa to Biomod/2012/TeamSendaiA/Methods in a maintenance job.)
     11:33  Biomod/2012/TU Dresden/Nanosaurs/Project/Sponsor‎ (diff | hist) . . (+1). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from Biomod/2012/TU Dresden/Nanosaurs/Sponsor to Biomod/2012/TU Dresden/Nanosaurs/Sponsors in a maintenance job.)
     11:33  User:Etienne Robillard/Notebook/Phenylmethylamine‎ (diff | hist) . . (-6). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from User:Etienne Robillard/Notebook/Phenylmethanamine to User:Etienne Robillard/Notebook/Benzylamine in a maintenance job.)
     11:33  Farre Lab:Teaching & Research Program‎ (diff | hist) . . (-18). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from Farre Lab:Teaching&Research Program to Farre Lab:NSF T&R in a maintenance job.)
     11:33  Farre Lab:Teaching&Research Program‎ (diff | hist) . . (-24). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from Farre Lab:Farre-Teaching&Research Program to Farre Lab:NSF T&R in a maintenance job.)
     11:33  Farre Lab:Farre-Teaching&Research Program‎ (diff | hist) . . (-21). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from Farre Lab:NSFTeaching&Research Program to Farre Lab:NSF T&R in a maintenance job.)
     11:33  User:Etienne Robillard/Notebook/Chemtrails911 notebook/Agent Ecoli:PhotoReceptorComponent‎ (diff | hist) . . (-26). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from User:Etienne Robillard/Notebook/Agent Ecoli:PhotoReceptorComponent to User:Etienne Robillard/Notebook/Agent BZ in a maintenance job.)
     11:33  BIOX‎ (diff | hist) . . (+9). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from Liu BioX to Template:Liu BioX in a maintenance job.)
     11:33  User:Kodipad Shafahed‎ (diff | hist) . . (+5). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from King jazz to User:King jazz in a maintenance job.)
     11:33  User talk:Kodipad Shafahed‎ (diff | hist) . . (+5). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from Talk:King jazz to User talk:King jazz in a maintenance job.)
     11:33  User talk:Sen Liu‎ (diff | hist) . . (+3). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from Talk:LiuGroup CTGU to Talk:LiuGroup of CTGU in a maintenance job.)
     11:33  User:Eung-Sam Kim‎ (diff | hist) . . (+1). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from Nanobilogy Lab to Nanobiology Lab in a maintenance job.)
     11:33  Talk:Eung-Sam Kim‎ (diff | hist) . . (+1). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from Talk:Nanobilogy Lab to Talk:Nanobiology Lab in a maintenance job.)
     11:33  Tnt - Labbook/2011/09/14‎ (diff | hist) . . (+1). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from Biomod/2011/TUM/TNT/Labbook/2011/09/14 to Biomod/2011/TUM/TNT/LabbookA/2011/09/14 in a maintenance job.)