Difference between revisions of "User:S Sibert/Notebook/SBB13Ntbk-Stephanie J Sibert"

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sequence confirmed<br>
golden gate<br>
6.5ul ddH2o<br>
1ul ligase buffer<br>
0.5ul ligase<br>
0.5ul BsMb1<br>
1ul of synthon mic<br>
o.5ul vector<br>
1 tube<br>
20ul KCM<br>
no h20<br>
Rescue <br>
6.5ul H20<br>
2ul Plasmid<br>
1ul NEB2<br>
0.5ul Bsa1<br>
lanes 7,8,9 <br>
A,B,D respectivly<br>
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Revision as of 14:20, 1 December 2013

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