Difference between revisions of "User:S Sibert/Notebook/SBB13Ntbk-Stephanie J Sibert"

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==4/19/2012==
 
sequence confirmed<br>
 
golden gate<br>
 
Protocol:<br>
 
6.5ul ddH2o<br>
 
1ul ligase buffer<br>
 
0.5ul ligase<br>
 
0.5ul BsMb1<br>
 
1ul of synthon mic<br>
 
o.5ul vector<br>
 
 
==4/23/13==
 
 
Transform<br>
 
1 tube<br>
 
20ul KCM<br>
 
no h20<br>
 
Rescue <br>
 
plate<br>
 
 
==4/25/13==
 
~miniprep~<br>
 
6.5ul H20<br>
 
2ul Plasmid<br>
 
1ul NEB2<br>
 
0.5ul Bsa1<br>
 
<Br>
 
~map~<br>
 
lanes 7,8,9 <br>
 
A,B,D respectivly<br>
 
 
 
 
 
~seq~<br>
 
 
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Revision as of 14:20, 1 December 2013

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