Difference between revisions of "User:Ryan T. Willett"

From OpenWetWare
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*molecular biology
*molecular biology
*RNA/DNA/protein biochemistry
*RNA/DNA/protein biochemistry
*cell culture and generation of stable cell lines
*cell culture and generation of stably transfected cell lines
*dissection and primary cell culture
*dissection and primary cell culture
*animal surgery
*rodent survival surgery
*mouse genetics
* ''in utero'' electroporation of DNA into rat embryos
* ''in utero'' electroporation of DNA into rat embryos
*pseudotype recombinant retrovirus production and purification
*pseudotyped recombinant retrovirus production and purification
==Research interests==
==Research interests==

Revision as of 15:24, 3 November 2011

Ryan T. Willett

I am a starting up my postdoc in the laboratory of Alexandra Joyner at MSKCC. Our lab is interested in developmental and pathological processes regulated by Sonic Hedgehog (SHH) signaling and in how the morphology, cytoarchitecture, cell fate specification, and circuitry formation of the cerebellum is achieved. My project, in its beginning stages, will focus on characterization of the function of the Engrailed family of homeodomain transcription factors, identification of gene targets that they act upon during transcriptional regulation, and identification of protein cofactors that they bind with to carry out this function.

OpenWetWare caught my attention in a writeup on DIY, howebrew biology in New Scientist magazine.

Contact Info

  • Laboratory of Alexandra Joyner
  • Sloan-Kettering Institute - Developmental Biology Program
  • New York, NY, USA
  • Contact Information


  • 2010 PhD, Columbia University
  • 2002 BS, Brandeis University - Biology
  • 2002 BA, Brandeis University - Biochemistry


  • Brian Hoffman Award for Excellence in Graduate Studies
  • Graduated with High Honors in Biology for the undergraduate thesis "Posttranscriptional Analysis of Circadian Rhythms" in the laboratory of Michael Rosbash


  • molecular biology
  • RNA/DNA/protein biochemistry
  • cell culture and generation of stably transfected cell lines
  • dissection and primary cell culture
  • rodent survival surgery
  • mouse genetics
  • in utero electroporation of DNA into rat embryos
  • pseudotyped recombinant retrovirus production and purification
  • histology/immunohistology/immunofluorescence

Research interests

  1. Neural Development
  2. Stem cell biology
  3. Cancer biology
  4. Genetic Engineering / Gene Therapy
  5. Bioengineering
  6. Biochemistry
  7. Bioinformatics


Biswas SC, Zhang Y, Iyirhiaro G, Willett RT, Rodriguez-Gonzalez Y, Cregan SP, Slack RS, Park DS, Greene LA. (2010) "SERTAD1 Plays an Essential Role in Developmental and Pathological Neuron Death". J Neurosci 30(11):3973-3982

Malagelada C, Lopez-Toledano MA, Willett RT, Jin ZH, Shelanski ML, Greene LA. (2011) Role of RTP801 in Neural Stem Cell Maintenance and Differentiation. J Neurosci 31(9):3186-96

Willett RT and Greene LA. (2011) "Gata2 is Required for Migration and Differentiation of Retinorecipient Neurons in the Superior Colliculus". J Neurosci 31(12):4444-55

My Links