User:Robwarden/Notebook/GFP-RGD Force Sensor

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Goal

Create a GFP-based sensor to measure the forces involved in cell motility.

Project Outline

  1. Express GFPuv with a C-terminal sortase tag.
  2. Replace two of GFP's β-barrel loops with fibronectin's RGD and synergy (PPSRN) loops.
  3. Coat PEG/PMMA gels with expressed GFP-RGD.
  4. Determine a force/fluorescence curve.
  5. Measure cellular motility forces in vivo.



Recent changes

26 September 2017

     15:27 

(Upload log). .

[Sayyed Ourmazd Mohseni‎; Sarah R. Brady‎ (4×); Pauline Wonnenberg‎ (4×)]

     

15:27

. . Pauline Wonnenberg (talk | contribs) uploaded File:Titration 3 Graph.png

     

14:11

. . Sarah R. Brady (talk | contribs) uploaded File:Heart rate with asterisk.jpg

     

14:03

. . Pauline Wonnenberg (talk | contribs) uploaded a new version of File:Titration 2 Graph.png

     

14:03

. . Pauline Wonnenberg (talk | contribs) uploaded File:Titration 2 Graph.png

     

14:02

. . Sarah R. Brady (talk | contribs) uploaded File:Temp with asterisk.jpg

     

14:01

. . Pauline Wonnenberg (talk | contribs) uploaded File:Titration 1 Graph.png

     

13:54

. . Sarah R. Brady (talk | contribs) uploaded File:Temp bme 100.jpg(temp bme 100 group 7)

     

13:54

. . Sarah R. Brady (talk | contribs) uploaded a new version of File:Temp.jpg(temp bme 100 group 7)

     

13:23

. . Sayyed Ourmazd Mohseni (talk | contribs) uploaded File:Heart Rate 22.png

     15:21  BioMicroCenter:BioinfoClasses‎ (diff | hist) . . (+64). . Vincent L. Butty (talk | contribs)

     14:42 

BME100 f2017:Group7 W1030 L3‎‎ (13 changes | history) . . (+310). . [Sarah R. Brady‎ (13×)]

     

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(cur | prev) . . (+243). . Sarah R. Brady (talk | contribs) (Experimental Design of Own Device)

     

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(cur | prev) . . (+226). . Sarah R. Brady (talk | contribs) (Experimental Design of Own Device)

     

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(cur | prev) . . (+21). . Sarah R. Brady (talk | contribs) (Design Flaws and Recommendations)

     

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(cur | prev) . . (+36). . Sarah R. Brady (talk | contribs) (Experimental Design of Own Device)

     

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(cur | prev) . . (+20). . Sarah R. Brady (talk | contribs) (Descriptive Stats)

     

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(cur | prev) . . (+82). . Sarah R. Brady (talk | contribs) (Experimental Design of Own Device)

     

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(cur | prev) . . (-270). . Sarah R. Brady (talk | contribs) (Design Flaws and Recommendations)

     

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(cur | prev) . . (-17). . Sarah R. Brady (talk | contribs) (Heart Rate Average)

     

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(cur | prev) . . (-1). . Sarah R. Brady (talk | contribs) (Temperature Average)

     

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(cur | prev) . . (-25). . Sarah R. Brady (talk | contribs) (Inferential Stats)

     

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(cur | prev) . . (+2). . Sarah R. Brady (talk | contribs) (Inferential Stats)

     

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(cur | prev) . . (+4). . Sarah R. Brady (talk | contribs) (Heart Rate Data Discussion)

     

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(cur | prev) . . (-11). . Sarah R. Brady (talk | contribs) (Inferential Stats)

 m   14:12  Talk:Butlin:Unix for Bioinformatics - advanced tutorial‎ (diff | hist) . . (-183). . Claudius E Kerth (talk | contribs)

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Butlin:Unix for Bioinformatics - advanced tutorial‎‎ (3 changes | history) . . (+648). . [Claudius E Kerth‎ (3×)]

 m   

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(cur | prev) . . (-158). . Claudius E Kerth (talk | contribs) (/* TASK 12: I need to run programme X that requires 2 input files and cannot read compressed files. My input files are very large and disk space is always precious. How can I avoid first having to uncompress my input files and later recompress them whe...)

     

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(cur | prev) . . (+804). . Claudius E Kerth (talk | contribs) (Added blurb to endnote, with link to Arnie's speech)

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(cur | prev) . . (+2). . Claudius E Kerth (talk | contribs) (Notes)

     14:00  User:Pauline Wonnenberg/Notebook/CHEM 471 Fall 2017/2017/09/06‎ (diff | hist) . . (+5). . Pauline Wonnenberg (talk | contribs)

     13:51 

Li Lab‎‎ (7 changes | history) . . (+3,624). . [Wei Li Bcm‎ (7×)]

     

13:51

(cur | prev) . . (+1). . Wei Li Bcm (talk | contribs)

     

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(cur | prev) . . (-39). . Wei Li Bcm (talk | contribs)

     

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(cur | prev) . . (-342). . Wei Li Bcm (talk | contribs)

     

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(cur | prev) . . (-104). . Wei Li Bcm (talk | contribs)

     

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(cur | prev) . . (+4). . Wei Li Bcm (talk | contribs)

     

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(cur | prev) . . (+3,825). . Wei Li Bcm (talk | contribs)

     

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(cur | prev) . . (+279). . Wei Li Bcm (talk | contribs)

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Butlin:Unix for Bioinformatics - basic tutorial‎‎ (2 changes | history) . . (+961). . [Claudius E Kerth‎ (2×)]

     

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(cur | prev) . . (0). . Claudius E Kerth (talk | contribs) (put definition of Unix to the front)

     

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(cur | prev) . . (+961). . Claudius E Kerth (talk | contribs) (Added appeal not to copy-paste command lines)

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Taylor:Publications‎‎ (4 changes | history) . . (+631). . [Lynne S. Taylor‎ (4×)]

     

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(cur | prev) . . (+316). . Lynne S. Taylor (talk | contribs) (PUBLICATIONS)

     

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(cur | prev) . . (-3). . Lynne S. Taylor (talk | contribs) (PUBLICATIONS)

     

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(cur | prev) . . (+58). . Lynne S. Taylor (talk | contribs) (PUBLICATIONS)

     

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(cur | prev) . . (+260). . Lynne S. Taylor (talk | contribs) (PUBLICATIONS)

     13:35  BME100 f2017:Group15 W1030 L3‎ (diff | hist) . . (+3). . Kylie I. Mercer (talk | contribs) (OUR TEAM)

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BME100 f2017:Group9 W0800 L3‎‎ (3 changes | history) . . (+2,231). . [Irene Zhang‎ (3×)]

     

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(cur | prev) . . (+1,582). . Irene Zhang (talk | contribs) (Design Flaws and Recommendations)

     

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(cur | prev) . . (+610). . Irene Zhang (talk | contribs) (Inferential Stats)

     

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(cur | prev) . . (+39). . Irene Zhang (talk | contribs) (Descriptive Stats and Graph)

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BME100 f2017:Group5 W0800 L3‎‎ (3 changes | history) . . (+2,331). . [Anna Hoge‎ (3×)]

     

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(cur | prev) . . (+160). . Anna Hoge (talk | contribs) (Inferential Stats and Analysis)

     

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(cur | prev) . . (+2,099). . Anna Hoge (talk | contribs) (Experimental Design of Own Device)

     

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(cur | prev) . . (+72). . Anna Hoge (talk | contribs) (Descriptive Stats and Graph)

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BME100 f2017:Group7 W0800 L3‎‎ (2 changes | history) . . (+26). . [Sayyed Ourmazd Mohseni‎; Miguel R. Almanza Lopez‎]

     

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(cur | prev) . . (-1). . Miguel R. Almanza Lopez (talk | contribs) (Design Flaws and Recommendations)

     

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(cur | prev) . . (+27). . Sayyed Ourmazd Mohseni (talk | contribs) (Descriptive Stats and Graph)