Difference between revisions of "User:Radoslaw Kwasniak/Notebook/Transcription factor delivery through T3SS/2012/07/11"

From OpenWetWare
Jump to: navigation, search
(1% Agarose Gel)
(Miniprep)
Line 16: Line 16:
 
* BBA_K11309 => 30μl DNA
 
* BBA_K11309 => 30μl DNA
  
μ==PCR - test==
+
==PCR - test==
 
Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))
 
Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))
  

Revision as of 11:01, 12 July 2012

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>

Overnight growth check

  • BBA_B0030 (good - turbid)
  • BBA_K11309 (good - turbid)

Miniprep

Used QIA Gen

  • BBA_B0030 => 30μl DNA
  • BBA_K11309 => 30μl DNA

PCR - test

Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))

Reaction

Conditions

1% Agarose Gel

Prepared: 0.65g of Agarose with 65ml of 1xTAE Gel-11-jul-2012.JPG Loading

  1. 1KB NEB Ladder - 10μL
  2. BBa_B0030 (RBS - undigested) - 5μL
  3. BBa_K113009 (PROMOTER ARA - undigested) - 5μL
  4. BLANK
  5. PCR katE, MASTER MIX (Ryhan)- 10μL
  6. BLANK
  7. PCR katE, Invitrogen - 50μL
  8. PCR katE, Invitrogen - 5μL