User:Radoslaw Kwasniak/Notebook/Transcription factor delivery through T3SS/2012/07/11: Difference between revisions
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* BBA_K11309 => 30μl DNA | * BBA_K11309 => 30μl DNA | ||
==PCR - test== | μ==PCR - test== | ||
Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan)) | Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan)) | ||
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* | * | ||
Conditions | Conditions | ||
==1% Agarose Gel== | |||
Prepared: 0.65g of Agarose with 65ml of 1xTAE | |||
Loading | |||
# 1KB NEB Ladder - 10μL | |||
# BBa_B0030 (RBS - undigested) - 5μL | |||
# BBa_K113009 (PROMOTER ARA - undigested) - 5μL | |||
# BLANK | |||
# PCR katE, MASTER MIX (Ryhan)- 10μL | |||
# BLANK | |||
# PCR katE, Invitrogen - 50μL | |||
# PCR katE, Invitrogen - 5μL | |||
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Revision as of 10:57, 12 July 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> |
Overnight growth check
MiniprepUsed QIA Gen
μ==PCR - test== Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan)) Reaction Conditions 1% Agarose GelPrepared: 0.65g of Agarose with 65ml of 1xTAE Loading
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