Difference between revisions of "User:Radoslaw Kwasniak/Notebook/Transcription factor delivery through T3SS/2012/07/11"

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(Miniprep)
(PCR - test)
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* BBA_K11309 => 30μl DNA
 
* BBA_K11309 => 30μl DNA
  
==PCR - test==
+
μ==PCR - test==
 
Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))
 
Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))
  
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*  
 
*  
 
Conditions
 
Conditions
 +
 +
==1% Agarose Gel==
 +
 +
Prepared: 0.65g of Agarose with 65ml of 1xTAE
 +
 +
Loading
 +
 +
# 1KB NEB Ladder  - 10μL
 +
# BBa_B0030 (RBS - undigested) - 5μL
 +
# BBa_K113009 (PROMOTER ARA - undigested) - 5μL
 +
# BLANK
 +
# PCR katE,  MASTER MIX (Ryhan)- 10μL
 +
# BLANK
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# PCR katE, Invitrogen - 50μL
 +
# PCR katE, Invitrogen - 5μL
 +
  
 
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Revision as of 09:57, 12 July 2012

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Overnight growth check

  • BBA_B0030 (good - turbid)
  • BBA_K11309 (good - turbid)

Miniprep

Used QIA Gen

  • BBA_B0030 => 30μl DNA
  • BBA_K11309 => 30μl DNA

μ==PCR - test== Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))

Reaction

Conditions

1% Agarose Gel

Prepared: 0.65g of Agarose with 65ml of 1xTAE

Loading

  1. 1KB NEB Ladder - 10μL
  2. BBa_B0030 (RBS - undigested) - 5μL
  3. BBa_K113009 (PROMOTER ARA - undigested) - 5μL
  4. BLANK
  5. PCR katE, MASTER MIX (Ryhan)- 10μL
  6. BLANK
  7. PCR katE, Invitrogen - 50μL
  8. PCR katE, Invitrogen - 5μL