Difference between revisions of "User:Radoslaw Kwasniak/Notebook/Transcription factor delivery through T3SS/2012/07/11"

From OpenWetWare
Jump to: navigation, search
(Overnight growth check)
(Miniprep)
Line 16: Line 16:
 
* BBA_K11309 => 30μl DNA
 
* BBA_K11309 => 30μl DNA
  
 +
==PCR - test==
 +
Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))
  
 +
Reaction
 +
*
 +
Conditions
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 09:53, 12 July 2012

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>

Overnight growth check

  • BBA_B0030 (good - turbid)
  • BBA_K11309 (good - turbid)

Miniprep

Used QIA Gen

  • BBA_B0030 => 30μl DNA
  • BBA_K11309 => 30μl DNA

PCR - test

Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))

Reaction

Conditions