User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/27: Difference between revisions
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== | ==Kinetics and Dialysis== | ||
* | |||
==Goals== | |||
* Make Au and ADA solutions with the ADA that was placed through dialysis [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/14| two weeks ago]] | |||
* Run Completed ADA kinetic assay using Newfrac ADA | |||
==Procedure== | |||
* The contents of the dialysis pouches were collected in separate falcon tubes. The two samples of ADA that were run through the dialysis were Frac #2 and Frac #8 from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]]. We decided to made the new Au/ADA solutions using ADA frac # 8. We chose it because after New frac, it was the collected fraction that had the highest [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/07| concentration of ADA present]]. | |||
* [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C. | |||
* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine. | |||
Revision as of 12:37, 27 November 2012
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Kinetics and DialysisGoals
Procedure
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