User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/13: Difference between revisions
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* The Adenosine Deaminase Kinetic assay was done by following the Assay [[AU Biomaterials Design Lab:Protocols/ADA Activity Assay| Protocol]] | * The Adenosine Deaminase Kinetic assay was done by following the Assay [[AU Biomaterials Design Lab:Protocols/ADA Activity Assay| Protocol]] | ||
# The potassium Phosphate Buffer was actually sodium phosphate and was made at a pH of 7.4 | |||
#* We made 50mL of .05M sodium phosphate buffer | |||
.05 x .05= .0025 moles | |||
.0025 moles x 268.07g/1mol = .6702g sodium phosphate in 50 mL H<sub>2</sub>0. Actual amount = .6705. pH of 7.4 was achieve by adding roughly 3 drops of 12M HCl from a glass pasture pipette. | |||
#* | |||
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Revision as of 11:10, 15 November 2012
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ADA Kinetics and moreGoals
Procedure
.05 x .05= .0025 moles .0025 moles x 268.07g/1mol = .6702g sodium phosphate in 50 mL H20. Actual amount = .6705. pH of 7.4 was achieve by adding roughly 3 drops of 12M HCl from a glass pasture pipette. |