User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/07: Difference between revisions
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==Goals== | ==Goals== | ||
* Conduct a bradford assay on the collected fraction of ADA made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06| 2012/11/06]] and on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]]. | * Conduct a bradford assay on the collected fraction of ADA made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06| 2012/11/06]] and on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]]. | ||
*Prepare Au/HRP solutions | |||
==Procedure== | ==Procedure== | ||
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* In a cuvet, 7.5μL of autclaved water, 7.5 μL of a fraction and 715μL 1X bradford Reagent were added. In total 3 cuvet samples were run through the UV-Vis. | * In a cuvet, 7.5μL of autclaved water, 7.5 μL of a fraction and 715μL 1X bradford Reagent were added. In total 3 cuvet samples were run through the UV-Vis. | ||
**Because the spectra for frac #8 and Frac New had an absorbance of over 1, those cuvet samples were diluted in half using the same procedure as for the standard solutions in order to get an acceptable (below 1) absorbance) | **Because the spectra for frac #8 and Frac New had an absorbance of over 1, those cuvet samples were diluted in half using the same procedure as for the standard solutions in order to get an acceptable (below 1) absorbance) | ||
* For all information regarding the Au/HRP solutions, please see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/07| Dhea's Notebook]] | |||
==DATA== | ==DATA== | ||
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Concentrations, absorbances, for the collected fracs of ADA | Concentrations, absorbances, for the collected fracs of ADA | ||
Sample Name Volume of ADA[mL] Absorbance [ADA] [mg/mL] Mass of ADA[mg] Mols of ADA [mol] Molar absorptivity [mL/mg*cm-1] molarity[M] uM | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Sample Name''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume of ADA[mL]''' | |||
| align="center" style="background:#f0f0f0;"|'''Absorbance''' | |||
| align="center" style="background:#f0f0f0;"|'''[ADA] [mg/mL]''' | |||
| align="center" style="background:#f0f0f0;"|'''Mass of ADA[mg]''' | |||
| align="center" style="background:#f0f0f0;"|'''Mols of ADA [mol]''' | |||
| align="center" style="background:#f0f0f0;"|'''Molar absorptivity [mL/mg*cm-1]''' | |||
| align="center" style="background:#f0f0f0;"|'''molarity[M]''' | |||
| align="center" style="background:#f0f0f0;"|'''uM''' | |||
|- | |||
| Frac 2||0.015||0.93||0.017747193||0.025644694||6.30091E-10||52.40265306||4.20061E-05||42.0060507 | |||
|- | |||
| Frac 8||0.0075||0.757||0.011481347||0.016590547||4.0763E-10||65.93302839||5.43507E-05||54.35068601 | |||
|- | |||
| Frac new||0.0075||0.819||0.013726911||0.019835386||4.87356E-10||59.66382586||6.49808E-05||64.98078863 | |||
|} | |||
Revision as of 11:03, 15 November 2012
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Bradford AssayGoals
Procedure
DATA
Concentrations, absorbances, for the collected fracs of ADA
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