User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06: Difference between revisions
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==Goals== | ==Goals== | ||
*Run Fast Protein Liquid Chromatography (FPLC) | *Run Fast Protein Liquid Chromatography (FPLC) | ||
* | *Redo cell transformation | ||
==Procedure== | |||
* Please refer to the [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| protocol]] that was previously employed. No changes were made in this procedure. 3 fracs of ADA were collected. Frac 2 had the most protein present with small amounts present in fracs 1 and 3. | |||
* The transformation and plating of PCR product that was done on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/24| 2012/10/24]] was unsuccessful and had to be repeated. the Original protocol for PCR mutation can be found [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol| here]]. The following modifications were made: | |||
**20μL DNA solution was added to 30μL solution with E. Coli cells. | |||
**250μL Lysogeny Broth (LB) was used instead of SOC medium. | |||
**Single 200μL plates were prepared. No 50μL plates were made. | |||
==Conclusions== | |||
Revision as of 14:19, 11 November 2012
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FPLC and TransformationGoals
Procedure
Conclusions |