User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/31: Difference between revisions
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==Procedure== | ==Procedure== | ||
*The same procedure from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/25| 2012/09/25]] was followed in order to separate out the proteins from the cell organelles. | *The same procedure from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/25| 2012/09/25]] was followed in order to separate out the proteins from the cell organelles. | ||
* For information regarding the AuNP lysozyme UV-Vis spectras please see [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/31| Michael's Notebook]]. | ** The cells were shocked open by alternating sonication for 30 seconds followed by 30 seconds on ice. | ||
**Afterwards, the cells were centrifuged at 18000 rpm and 4°C for 2 hours. | |||
**The supernatant was collected and purified through vacuum filtration with filter pape membrane in which the holes measure 450nm. The filtered product was collected and stored in the refrigerator for approximately 1 week. | |||
* For information regarding the AuNP lysozyme UV-Vis spectras please see [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/31| Michael's Notebook]]. These spectras were taken using the same samples as were made [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/24| last week]] | |||
==Data== | ==Data== |
Revision as of 12:32, 24 November 2012
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Separate Protein and Lysozyme UV-VisGoals
Procedure
DataConclusions
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