User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/17

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Gel Electrophoresis

Goals

  • Run the plasmids through a gel electrophoresis.
  • Complete HRP chemiluminescence assay

Procedure

Conclusions

  • The Fluorimeter was not working today and attempts to use the Uv-Vis in a makeshift manner in order to calculate the luminescence emitted were unsuccessful. As a result this experiment has been placed on hold until the equipment is once again working.
  • The duration of lab was used to make new Au/BSA solutions at the same original 14 concentrations and the lysozyme stock solution was also made. The lysozyme will be used to nucleate the AuNPs in the future. I was not present in lab during this time. For information regarding the making of the Au/BSA solutions please consult Michaels Notebook. For instructions and calculations regarding the lysozyme stock please look at Mary's Notebook.