User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/09: Difference between revisions

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==Procedure==
==Procedure==
* The AA was calibrated using standard solutions made by [[User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08| Dr. Miller]]. The concentrations of these standard solutions were 5,8,10,15,20,25,30 and 40 ppm. The test was also blanked with 1M HCl.  
* The AA was calibrated using standard solutions made by [[User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08| Dr. Miller]]. The concentrations of these standard solutions were 5,8,10,15,20,25,30 and 40 ppm. The AA runs were blanked with 1M HCl.  
*Deionized water was run through the AA between samples.  
*Deionized water was run through the AA between samples.  
* The Au/BSA samples had been made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]].  
* The Au/BSA samples had been made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]].  
*The samples were runt through the AA and blanked with 1M HCl between every 4 samples.  
*The samples were runt through the AA and blanked with 1M HCl between every 4 samples.


==Data/Results==
==Data/Results==
Line 79: Line 79:


* Absorbance and concentrations of standards and solutions. It can be seen that there is a general trend. as concentration increases, so does absorbance. There is a drop and then a spike and the 138-140 range.  
* Absorbance and concentrations of standards and solutions. It can be seen that there is a general trend. as concentration increases, so does absorbance. There is a drop and then a spike and the 138-140 range.  
[[Image:AA first round.png| AA data]]
[[Image:AASRound1.png]]
 
 
[[Image:AA calibration cuve.png| AA calibration curves]]


==Conclusions==
==Conclusions==
*For unknown reasons, there were no fibers present in the solution. But it was apparent that the gold had reacted because the solution was purple. Therefore, we went ahead with the experiment with the intent or repeating it at a later date.  
*For unknown reasons, there were no fibers present in the solution. But it was apparent that the gold had reacted because the solution was purple. Therefore, we went ahead with the experiment with the intent or repeating it at a later date.  
* The AAS allowed us to conclude that the measured concentration of gold in the solution was different from what we had anticipated when we made the solutions at specific mole ratios. This could indicate the fact that the lack of NP fibers in these samples resulted in the variation in concentration observed.




Revision as of 16:32, 21 November 2012

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Atomic Absorption Spectrum

Goals

  • Run AAS on AuNP/BSA samples to determine true concentrations

Procedure

  • The AA was calibrated using standard solutions made by Dr. Miller. The concentrations of these standard solutions were 5,8,10,15,20,25,30 and 40 ppm. The AA runs were blanked with 1M HCl.
  • Deionized water was run through the AA between samples.
  • The Au/BSA samples had been made on 2012/09/26.
  • The samples were runt through the AA and blanked with 1M HCl between every 4 samples.

Data/Results

Standard True Value Concentration Absorbance
5 - 0.0913
8 - 0.1547
10 - 0.1993
15 - 0.3175
20 - 0.4059
25 - 0.4918
30 - 0.5928
40 - 0.7299
Blank - 0.0198


Sample Concentration Absorbance
60 11.0972 0.2228
80 15.3973 0.3028
100 21.6271 0.4187
120 21.7669 0.4213
128 22.4442 0.4339
130 23.7718 0.4586
132 21.3584 0.4137
133 23.0515 0.4452
134 19.1492 0.3726
136 25.3629 0.4882
138 24.6318 0.4746
140 24.2126 0.4668
160 27.2603 0.5235
170 29.3405 0.5622


  • Absorbance and concentrations of standards and solutions. It can be seen that there is a general trend. as concentration increases, so does absorbance. There is a drop and then a spike and the 138-140 range.


AA calibration curves

Conclusions

  • For unknown reasons, there were no fibers present in the solution. But it was apparent that the gold had reacted because the solution was purple. Therefore, we went ahead with the experiment with the intent or repeating it at a later date.
  • The AAS allowed us to conclude that the measured concentration of gold in the solution was different from what we had anticipated when we made the solutions at specific mole ratios. This could indicate the fact that the lack of NP fibers in these samples resulted in the variation in concentration observed.



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