User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/19: Difference between revisions
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# Inoculate the [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture media]] by dividing the resuspended cells among the Fernbach flasks. | # Inoculate the [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture media]] by dividing the resuspended cells among the Fernbach flasks. | ||
--[[User:Abigail E. Miller|Abigail E. Miller]] 10:53, 7 October 2012 (EDT)it is not clear that this was done in the morning. | --[[User:Abigail E. Miller|Abigail E. Miller]] 10:53, 7 October 2012 (EDT)it is not clear that this was done in the morning. | ||
That afternoon: | |||
# | # 1mL of 0.4M [[AU_Biomaterials_Design_Lab:Materials/IPTG|IPTG]] was added to each flask in order to induce protein expression. The 4 fernbach flasks were then allowed to continue to shake for 3 more hours. | ||
#The cells were harvested by centrifuging at 4500rpm for 15 minutes. 2 runs of this had to be done because not all the media was able to fit in the centrifuge jars in one run. | |||
# | # The cells were then resuspended using 30mL of binding buffer for flasks 2-4 and 10mL separate for flask 1. | ||
# These flasks were then placed in a -80°C freezer overnight. | |||
==Calculations== | ==Calculations== |
Revision as of 12:01, 24 November 2012
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Protein Expression.Goals
Procedure
--Abigail E. Miller 10:53, 7 October 2012 (EDT)it is not clear that this was done in the morning. That afternoon:
Calculations
a. Theoretical calculations
a. Theoretical calculations
--Abigail E. Miller 10:54, 7 October 2012 (EDT)did you have to adjust the pH? Conclusions
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