User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18

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Day 5

  • Protein Expressions and Starters

Goals

  • Grow E.Coli cells and have them express ADA protein.
  • Follow Protocols for making starter and expression cultures of the cells.

Procedure

(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)


(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)


  • Next, two of the erlenmeyer flasks and two of the Fernbach flasks were placed in the Autoclave at a temperature of 121°C for 60minutes followed by a 30 minute cool down period.