# User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/11

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## Goals

• Run UV-Vis on AuNP/BSA samples 120, 128, 130, 132, 133, 134. and see if similar peaks are present
• Run third UV-Vis on Tris buffer solutions of pH 8 and compare absorbances to the data collected last week.

## Procedure

• UV-Vis was run for the AuNP/BSA samples as well as water to account for our blank.
1. UV/Vis was run on HAuCl4 and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134.
1. The same cuvette was used and cleaned between each spectra.
• The third UV-Vis was run on the Tris buffer solutions at pH 8. The first two rounds were run the previous time.
• More AuNP/ BSA solution was made at a concentration of 170 and place in the oven for 4 hours at 80°C. These samples are to be used tomorrow and will be combined with the pH 10 tris buffer solution and have a UV-Vis run on them every hour.
• Abigail E. Miller 13:50, 17 September 2012 (EDT):check your references to HAuCl4 and AuNPS.

## Data/ Calculations

1. Solution was made with a mole ratio of 170
1. 2.4268M BSA solution was used
1. m1v1=m2v2
2. 24.266μM * (volume) = 1μM * 6mL
3. .247mL BSA solution
2. 2150μM HAuCl4 solution was used
1. 2150μM * (volume) = 6mL *(1μM*170)
2. .474mL HAuCl4 solution
• 7 solutions in total were made to be used tomorrow.

Redone UV=Vis

Redone Uv-vis minus water abs.

• A UV-Vis was run for water and then those abs values were subtracted from the UV-Vis absorbance values collected by the samples. This allowed for us to account for a blank in the peak.