Difference between revisions of "User:Puja Mody/Notebook/AU Biomaterials 572/2014/04/16"

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(DATA)
(DATA)
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| 10||83||0.193022033||0.000193022||||
 
| 10||83||0.193022033||0.000193022||||
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Table 3: AA data for both gold and Iron Lamps
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Sample number'''
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| align="center" style="background:#f0f0f0;"|'''Sample name'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Gold in sample (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Total concentration Gold originally in solution (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Concentration gold incorporated (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Percent Gold incorporated (%)'''
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|-
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| 1||13500:1 Mag:BSA N2 prep with Au||0.04467344||1765.35||1765.305327||99.99746943
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|-
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| 2||13500:1 Mag:BSA:Au sonicated||0.0464768||1765.35||1765.303523||99.99736728
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|-
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| 3||13000:1 Mag:BSA:Au oven||0.04685952||1765.35||1765.30314||99.9973456
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| 4||16500:1 Mag:Heme:Au oven||0.04447792||1765.35||1765.305522||99.9974805
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|-
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| 5||16500:1 Mag:Heme:Au sonicated||0.04400784||1765.35||1765.305992||99.99750713
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| ||||||||||
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|-
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| Sample number||Sample name||Concentration Iron in sample (ug/mL)||Total concentration Iron originally in solution (ug/mL)||Concentration Iron incorporated (ug/mL)||Percent Iron incorporated (%)
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|-
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| 1||13500:1 Mag:BSA N2 prep||0.022256152||1765.35||1765.327744||99.99873928
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|-
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| 2||13500:1 Mag:BSA N2 prep with Au||0.022219961||1765.35||1765.32778||99.99874133
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|-
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| 3||13000:1 Mag:BSA oven||0.02224606||1765.35||1765.327754||99.99873985
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|-
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| 4||16500:1 Mag:Heme oven||0.02224084||1765.35||1765.327759||99.99874015
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|-
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| 5||16500:1 Mag:Heme:Au oven||0.022238231||1765.35||1765.327762||99.99874029
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|-
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| 6||16500:1 Mag:Heme:Au sonicated||0.022220831||1765.35||1765.327779||99.99874128
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|-
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| 7||13500:1 Mag:BSA:Au sonicated||0.022207781||1765.35||1765.327792||99.99874202
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|-
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| 8||13000:1 Mag:BSA:Au oven||0.022180812||1765.35||1765.327819||99.99874355
 
|}
 
|}

Revision as of 16:56, 28 April 2014

Goals

  • View samples of Magnetite-Protein (made in oven) as well as magnetite- protein-gold samples made in the oven and via sonication.
  • Run DSC of samples dried out yesterday
  1. 13500 mag BSA gold N2 prep
  2. 13500 mag-BSA N2 prep
  • Run AA of remaining necessary samples (both Gold and Fe)

DATA

Table 1: Sonicated samples microscope data Sample Name 165 mag heme gold sonicated 2.5x Particle number pixel size actual size (mm) Actual size (m) Average 1 34 0.079069767 7.90698E-05 9.81395E-05 98.13953488 2 53 0.123255814 0.000123256 3 26 0.060465116 6.04651E-05 STD DEV 4 46 0.106976744 0.000106977 1.75338E-05 17.53378018 5 43 0.1 0.0001 6 46 0.106976744 0.000106977 7 47 0.109302326 0.000109302 8 42 0.097674419 9.76744E-05 9 40 0.093023256 9.30233E-05 10 45 0.104651163 0.000104651

Sample name 130 mag bsa gold sonicated 2.5 x Particle number pixel size actual size (mm) Actual size (m) Average 1 32 0.074418605 7.44186E-05 8.27907E-05 82.79069767 2 43 0.1 0.0001 3 37 0.086046512 8.60465E-05 STD DEV 4 33 0.076744186 7.67442E-05 1.07526E-05 10.75258349 5 30 0.069767442 6.97674E-05 6 35 0.081395349 8.13953E-05 7 37 0.086046512 8.60465E-05 8 40 0.093023256 9.30233E-05 9 29 0.06744186 6.74419E-05 10 40 0.093023256 9.30233E-05

Table 2: Oven prep samples microscope data

Sample name 165 mag heme oven ' ' ' '
Particle number pixel size actual size (mm) Actual size (m) Average in um
1 90 0.209302326 0.000209302 0.000163123 163.1229236
2 66 0.153488372 0.000153488
3 65 0.151162791 0.000151163 STD DEV
4 67 0.155813953 0.000155814 2.25645E-05 22.56447491
5 61 0.141860465 0.00014186
6 67 0.155813953 0.000155814
7 75 0.174418605 0.000174419
Fiber width range pixel size actual size (mm) actual size (m) average in um
1 30 0.069767442 6.97674E-05 0.000202093 202.0930233
2 110 0.255813953 0.000255814
3 181 0.420930233 0.00042093 STD DEV
4 12 0.027906977 2.7907E-05 0.000160233 160.2325769
5 81 0.188372093 0.000188372
6 23 0.053488372 5.34884E-05
7 147 0.341860465 0.00034186
8 39 0.090697674 9.06977E-05
9 46 0.106976744 0.000106977
10 200 0.465116279 0.000465116
Sample Name 130 mag heme oven
Particle number pixel size actual size (mm) actual size (m) average in um
1 56 0.130231733 0.000130232 0.000295347 295.3469667
2 226 0.525578067 0.000525578
3 137 0.318602633 0.000318603
4 49 0.113952767 0.000113953 STD DEV
5 200 0.465113333 0.000465113 0.000165655 165.6548003
6 222 0.5162758 0.000516276
7 132 0.3069748 0.000306975
8 49 0.113952767 0.000113953
9 63 0.1465107 0.000146511
10 136 0.316277067 0.000316277
Fiber width range pixel size actual size (mm) actual size (m) average in um
1 106 0.246510067 0.00024651 0.000219932 219.9321619
2 185 0.430229833 0.00043023
3 79 0.183719767 0.00018372 STD DEV
4 116 0.269765733 0.000269766 0.000110007 110.0073921
5 76 0.176743067 0.000176743
6 57 0.1325573 0.000132557
7 43 0.099999367 9.99994E-05
Sample Name 135 mag BSA oven gold
Particle number pixel size actual size (mm) actual size (m) average in um
1 102 0.2372078 0.000237208 0.000202092 202.0917433
2 85 0.197673167 0.000197673
3 70 0.162789667 0.00016279
4 132 0.3069748 0.000306975 STD DEV
5 83 0.193022033 0.000193022 8.66719E-05 86.67187257
6 55 0.127906167 0.000127906
7 30 0.069767 0.000069767
8 125 0.290695833 0.000290696
9 139 0.323253767 0.000323254
10 48 0.1116272 0.000111627
Sample Name 165 mag heme oven gold
Particle number pixel size actual size (mm) actual size (m) average in um
1 126 0.2930214 0.000293021 0.000253022 253.0216533
2 76 0.176743067 0.000176743
3 88 0.204649867 0.00020465 STD DEV
4 120 0.279068 0.000279068 5.73933E-05 57.39330175
5 83 0.193022033 0.000193022
6 146 0.339532733 0.000339533
7 134 0.311625933 0.000311626
8 123 0.2860447 0.000286045
9 109 0.253486767 0.000253487
10 83 0.193022033 0.000193022


Table 3: AA data for both gold and Iron Lamps

Sample number Sample name Concentration Gold in sample (ug/mL) Total concentration Gold originally in solution (ug/mL) Concentration gold incorporated (ug/mL) Percent Gold incorporated (%)
1 13500:1 Mag:BSA N2 prep with Au 0.04467344 1765.35 1765.305327 99.99746943
2 13500:1 Mag:BSA:Au sonicated 0.0464768 1765.35 1765.303523 99.99736728
3 13000:1 Mag:BSA:Au oven 0.04685952 1765.35 1765.30314 99.9973456
4 16500:1 Mag:Heme:Au oven 0.04447792 1765.35 1765.305522 99.9974805
5 16500:1 Mag:Heme:Au sonicated 0.04400784 1765.35 1765.305992 99.99750713
Sample number Sample name Concentration Iron in sample (ug/mL) Total concentration Iron originally in solution (ug/mL) Concentration Iron incorporated (ug/mL) Percent Iron incorporated (%)
1 13500:1 Mag:BSA N2 prep 0.022256152 1765.35 1765.327744 99.99873928
2 13500:1 Mag:BSA N2 prep with Au 0.022219961 1765.35 1765.32778 99.99874133
3 13000:1 Mag:BSA oven 0.02224606 1765.35 1765.327754 99.99873985
4 16500:1 Mag:Heme oven 0.02224084 1765.35 1765.327759 99.99874015
5 16500:1 Mag:Heme:Au oven 0.022238231 1765.35 1765.327762 99.99874029
6 16500:1 Mag:Heme:Au sonicated 0.022220831 1765.35 1765.327779 99.99874128
7 13500:1 Mag:BSA:Au sonicated 0.022207781 1765.35 1765.327792 99.99874202
8 13000:1 Mag:BSA:Au oven 0.022180812 1765.35 1765.327819 99.99874355