Difference between revisions of "User:Puja Mody/Notebook/AU Biomaterials 572/2014/04/16"

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==Goals==
+
{{deleteme}}
*View samples of Magnetite-Protein (made in oven) as well as magnetite- protein-gold samples made in the oven and via sonication.
 
*Run DSC of samples dried out yesterday
 
#13500 mag BSA gold N2 prep
 
#13500 mag-BSA N2 prep
 
*Run AA of remaining necessary samples (both Gold and Fe)
 
 
 
==DATA==
 
Table 1: Sonicated samples microscope data
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Sample Name'''
 
| align="center" style="background:#f0f0f0;"|'''165 mag heme gold sonicated 2.5x'''
 
| align="center" style="background:#f0f0f0;"|''''''
 
| align="center" style="background:#f0f0f0;"|''''''
 
| align="center" style="background:#f0f0f0;"|''''''
 
| align="center" style="background:#f0f0f0;"|''''''
 
|-
 
| Particle number||pixel size||actual size (mm)||Actual size (m)||Average||
 
|-
 
| 1||34||0.079069767||7.90698E-05||9.81395E-05||98.13953488
 
|-
 
| 2||53||0.123255814||0.000123256||||
 
|-
 
| 3||26||0.060465116||6.04651E-05||STD DEV||
 
|-
 
| 4||46||0.106976744||0.000106977||1.75338E-05||17.53378018
 
|-
 
| 5||43||0.1||0.0001||||
 
|-
 
| 6||46||0.106976744||0.000106977||||
 
|-
 
| 7||47||0.109302326||0.000109302||||
 
|-
 
| 8||42||0.097674419||9.76744E-05||||
 
|-
 
| 9||40||0.093023256||9.30233E-05||||
 
|-
 
| 10||45||0.104651163||0.000104651||||
 
|-
 
| ||||||||||
 
|-
 
| Sample name||130 mag bsa gold sonicated 2.5 x||||||||
 
|-
 
| Particle number||pixel size||actual size (mm)||Actual size (m)||Average||
 
|-
 
| 1||32||0.074418605||7.44186E-05||8.27907E-05||82.79069767
 
|-
 
| 2||43||0.1||0.0001||||
 
|-
 
| 3||37||0.086046512||8.60465E-05||STD DEV||
 
|-
 
| 4||33||0.076744186||7.67442E-05||1.07526E-05||10.75258349
 
|-
 
| 5||30||0.069767442||6.97674E-05||||
 
|-
 
| 6||35||0.081395349||8.13953E-05||||
 
|-
 
| 7||37||0.086046512||8.60465E-05||||
 
|-
 
| 8||40||0.093023256||9.30233E-05||||
 
|-
 
| 9||29||0.06744186||6.74419E-05||||
 
|-
 
| 10||40||0.093023256||9.30233E-05||||
 
|}
 
 
 
Table 2: Oven prep samples microscope data
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Sample name'''
 
| align="center" style="background:#f0f0f0;"|'''165 mag heme oven'''
 
| align="center" style="background:#f0f0f0;"|''''''
 
| align="center" style="background:#f0f0f0;"|''''''
 
| align="center" style="background:#f0f0f0;"|''''''
 
| align="center" style="background:#f0f0f0;"|''''''
 
|-
 
| Particle number||pixel size||actual size (mm)||Actual size (m)||Average||in um
 
|-
 
| 1||90||0.209302326||0.000209302||0.000163123||163.1229236
 
|-
 
| 2||66||0.153488372||0.000153488||||
 
|-
 
| 3||65||0.151162791||0.000151163||STD DEV||
 
|-
 
| 4||67||0.155813953||0.000155814||2.25645E-05||22.56447491
 
|-
 
| 5||61||0.141860465||0.00014186||||
 
|-
 
| 6||67||0.155813953||0.000155814||||
 
|-
 
| 7||75||0.174418605||0.000174419||||
 
|-
 
| ||||||||||
 
|-
 
| Fiber width range||pixel size ||actual size (mm)||actual size (m)||average||in um
 
|-
 
| 1||30||0.069767442||6.97674E-05||0.000202093||202.0930233
 
|-
 
| 2||110||0.255813953||0.000255814||||
 
|-
 
| 3||181||0.420930233||0.00042093||STD DEV||
 
|-
 
| 4||12||0.027906977||2.7907E-05||0.000160233||160.2325769
 
|-
 
| 5||81||0.188372093||0.000188372||||
 
|-
 
| 6||23||0.053488372||5.34884E-05||||
 
|-
 
| 7||147||0.341860465||0.00034186||||
 
|-
 
| 8||39||0.090697674||9.06977E-05||||
 
|-
 
| 9||46||0.106976744||0.000106977||||
 
|-
 
| 10||200||0.465116279||0.000465116||||
 
|-
 
| ||||||||||
 
|-
 
| ||||||||||
 
|-
 
| Sample Name||130 mag heme oven||||||||
 
|-
 
| Particle number ||pixel size ||actual size (mm)||actual size (m)||average||in um
 
|-
 
| 1||56||0.130231733||0.000130232||0.000295347||295.3469667
 
|-
 
| 2||226||0.525578067||0.000525578||||
 
|-
 
| 3||137||0.318602633||0.000318603||||
 
|-
 
| 4||49||0.113952767||0.000113953||STD DEV||
 
|-
 
| 5||200||0.465113333||0.000465113||0.000165655||165.6548003
 
|-
 
| 6||222||0.5162758||0.000516276||||
 
|-
 
| 7||132||0.3069748||0.000306975||||
 
|-
 
| 8||49||0.113952767||0.000113953||||
 
|-
 
| 9||63||0.1465107||0.000146511||||
 
|-
 
| 10||136||0.316277067||0.000316277||||
 
|-
 
| ||||||||||
 
|-
 
| Fiber width range||pixel size ||actual size (mm)||actual size (m)||average||in um
 
|-
 
| 1||106||0.246510067||0.00024651||0.000219932||219.9321619
 
|-
 
| 2||185||0.430229833||0.00043023||||
 
|-
 
| 3||79||0.183719767||0.00018372||STD DEV||
 
|-
 
| 4||116||0.269765733||0.000269766||0.000110007||110.0073921
 
|-
 
| 5||76||0.176743067||0.000176743||||
 
|-
 
| 6||57||0.1325573||0.000132557||||
 
|-
 
| 7||43||0.099999367||9.99994E-05||||
 
|-
 
| ||||||||||
 
|-
 
| ||||||||||
 
|-
 
| ||||||||||
 
|-
 
| Sample Name||135 mag BSA oven gold||||||||
 
|-
 
| Particle number ||pixel size ||actual size (mm)||actual size (m)||average||in um
 
|-
 
| 1||102||0.2372078||0.000237208||0.000202092||202.0917433
 
|-
 
| 2||85||0.197673167||0.000197673||||
 
|-
 
| 3||70||0.162789667||0.00016279||||
 
|-
 
| 4||132||0.3069748||0.000306975||STD DEV||
 
|-
 
| 5||83||0.193022033||0.000193022||8.66719E-05||86.67187257
 
|-
 
| 6||55||0.127906167||0.000127906||||
 
|-
 
| 7||30||0.069767||0.000069767||||
 
|-
 
| 8||125||0.290695833||0.000290696||||
 
|-
 
| 9||139||0.323253767||0.000323254||||
 
|-
 
| 10||48||0.1116272||0.000111627||||
 
|-
 
| ||||||||||
 
|-
 
| Sample Name||165 mag heme oven gold||||||||
 
|-
 
| Particle number ||pixel size ||actual size (mm)||actual size (m)||average||in um
 
|-
 
| 1||126||0.2930214||0.000293021||0.000253022||253.0216533
 
|-
 
| 2||76||0.176743067||0.000176743||||
 
|-
 
| 3||88||0.204649867||0.00020465||STD DEV||
 
|-
 
| 4||120||0.279068||0.000279068||5.73933E-05||57.39330175
 
|-
 
| 5||83||0.193022033||0.000193022||||
 
|-
 
| 6||146||0.339532733||0.000339533||||
 
|-
 
| 7||134||0.311625933||0.000311626||||
 
|-
 
| 8||123||0.2860447||0.000286045||||
 
|-
 
| 9||109||0.253486767||0.000253487||||
 
|-
 
| 10||83||0.193022033||0.000193022||||
 
|}
 
 
 
 
 
Table 3: AA data for both gold and Iron Lamps
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Sample number'''
 
| align="center" style="background:#f0f0f0;"|'''Sample name'''
 
| align="center" style="background:#f0f0f0;"|'''Concentration Gold in sample (ug/mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Total concentration Gold originally in solution (ug/mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Concentration gold incorporated (ug/mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Percent Gold incorporated (%)'''
 
|-
 
| 1||13500:1 Mag:BSA N2 prep with Au||0.04467344||1765.35||1765.305327||99.99746943
 
|-
 
| 2||13500:1 Mag:BSA:Au sonicated||0.0464768||1765.35||1765.303523||99.99736728
 
|-
 
| 3||13000:1 Mag:BSA:Au oven||0.04685952||1765.35||1765.30314||99.9973456
 
|-
 
| 4||16500:1 Mag:Heme:Au oven||0.04447792||1765.35||1765.305522||99.9974805
 
|-
 
| 5||16500:1 Mag:Heme:Au sonicated||0.04400784||1765.35||1765.305992||99.99750713
 
|-
 
| ||||||||||
 
|-
 
| Sample number||Sample name||Concentration Iron in sample (ug/mL)||Total concentration Iron originally in solution (ug/mL)||Concentration Iron incorporated (ug/mL)||Percent Iron incorporated (%)
 
|-
 
| 1||13500:1 Mag:BSA N2 prep||0.022256152||1765.35||1765.327744||99.99873928
 
|-
 
| 2||13500:1 Mag:BSA N2 prep with Au||0.022219961||1765.35||1765.32778||99.99874133
 
|-
 
| 3||13000:1 Mag:BSA oven||0.02224606||1765.35||1765.327754||99.99873985
 
|-
 
| 4||16500:1 Mag:Heme oven||0.02224084||1765.35||1765.327759||99.99874015
 
|-
 
| 5||16500:1 Mag:Heme:Au oven||0.022238231||1765.35||1765.327762||99.99874029
 
|-
 
| 6||16500:1 Mag:Heme:Au sonicated||0.022220831||1765.35||1765.327779||99.99874128
 
|-
 
| 7||13500:1 Mag:BSA:Au sonicated||0.022207781||1765.35||1765.327792||99.99874202
 
|-
 
| 8||13000:1 Mag:BSA:Au oven||0.022180812||1765.35||1765.327819||99.99874355
 
|}
 

Latest revision as of 17:13, 28 April 2014

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