Difference between revisions of "User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/19"

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(Procedure)
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==Day 8==
 
* Goals
 
#Finish running UV-Vis on samples from yesterday
 
# Make new Heme-Mag samples in petri dishes so that they may be analyzed using a microscope.
 
# Run FTIR for samples which were dried [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/18|yesterday]]
 
 
==Procedure==
 
* For making the samples in the petri dish, we followed the sample protocol as we use for oven prep except this time we made 20mL of sample.
 
 
Table 1. Preparation of FeSO<sub>4</sub>*7H<sub>2</sub>O stock solution
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
 
| align="center" style="background:#f0f0f0;"|''''''
 
|-
 
| Molecular Weight (g/mol)||278.01
 
|-
 
| mass(g)||2.085075
 
|-
 
| volume stock (L)||0.025
 
|-
 
| Concentration (M)||0.3
 
|}
 
<br>
 
Table 2. Preparation of KOH stock solution
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
 
| align="center" style="background:#f0f0f0;"|''''''
 
|-
 
| Molecular Weight (g/mol)||56.11
 
|-
 
| mass(g)||1.5
 
|-
 
| volume stock (L)||0.025
 
|-
 
| Concentration (M)||1.069328106
 
|}
 
<br>
 
Table 3. Preparation of KNO<sub>3</sub> stock solution
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
 
| align="center" style="background:#f0f0f0;"|''''''
 
|-
 
| Molecular Weight (g/mol)||101.1
 
|-
 
| mass(g)||9.099
 
|-
 
| volume stock (L)||0.1
 
|-
 
| Concentration (M)||0.9
 
|}
 
<br>
 
Table 4. Preparation of Hemoglobin stock solution
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Preparation of hemoglobin Stock solution'''
 
| align="center" style="background:#f0f0f0;"|''''''
 
|-
 
| Molecular weight (g/mol)||64500
 
|-
 
| mass hemglobin (g)||0.0995
 
|-
 
| volume solution (L)||0.1
 
|-
 
| Concentration (M)||1.54264E-05
 
|}
 
<br>
 
Table 5. Reaction Mixture preparation
 
<br>
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 
| align="center" style="background:#f0f0f0;"|'''concentration Hemoglobin (M)'''
 
| align="center" style="background:#f0f0f0;"|'''volume of Hemoglobin added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
 
| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
 
|-
 
| 12500||0.0000024||3.111557789||2||1.870333333||8.888888889||4.129219989
 
|-
 
| 13000||2.30769E-06||2.991882489||2||1.870333333||8.888888889||4.248895288
 
|-
 
| 13500||2.22222E-06||2.881072027||2||1.870333333||8.888888889||4.359705751
 
|-
 
| 14000||2.14286E-06||2.778176597||2||1.870333333||8.888888889||4.462601181
 
|-
 
| 14500||2.06897E-06||2.682377404||2||1.870333333||8.888888889||4.558400374
 
|-
 
| 15000||0.000002||2.592964824||2||1.870333333||8.888888889||4.647812954
 
|-
 
| 15500||1.93548E-06||2.509320798||2||1.870333333||8.888888889||4.73145698
 
|-
 
| 16000||0.000001875||2.430904523||2||1.870333333||8.888888889||4.809873255
 
|-
 
| 16500||1.81818E-06||2.357240749||2||1.870333333||8.888888889||4.883537029
 
|-
 
| 17000||1.76471E-06||2.287910139||2||1.870333333||8.888888889||4.952867639
 
|}
 
<br>
 
*These samples will be analyzed using a microscope next lab.
 
<br>
 
 
==Data==
 
*For FTIR and UV-Vis spectras collected today, please see [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/19|Moira's notebook]]
 
 
  
  

Revision as of 22:11, 17 April 2014

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