Difference between revisions of "User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/12"

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==Observations==
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* For the oven prep reaction mixtures. It was noticed that immediately after adding the  FeSO<sub>4</sub>*7H<sub>2</sub>O a dark(dark green/blue) precipitate in both small clusters and long string like shape formed in the Heme:Magnetite samples. We always add FeSO<sub>4</sub>*7H<sub>2</sub>O last into the solutions so that it does not prematurely react with the other compounds and to make sure it does not inhibit the reaction from proceeding.
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*Upon observing the BSA:Magnetite samples, it appeared that large clusters formed (leaf like structures) at the 12500:1 ratio and continued to form up to the 15000:1 ratio. Prior to the 12500:1 ratio, it appears a magnetic colloid suspension was formed, indicating that all ratios successfully formed magnetite nanoparticles. However, for potential fiber formation, it appears the 12500:1-15000:1 range has potential.
  
 
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Revision as of 11:22, 24 February 2014

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Day 6

  • Goals
    • Prepare hemoglobin-magnetite solutions at the new ratios in the oven for comparison to the ones made of BSA. The BSA- Magnetite reaction mixtures at these ratios appeared to have more fiber like precipitates.
    • UV-Vis the samples prepared on Yesterday
    • Separate out the magnetite nanoparticles/ fibers using a magnet from the solutions prepared Yesterday for future use in FTIR and XRD analysis
    • Analyze the magnetite nanoparticles/ fibers separated Yesterday using FTIR.

Procedure

  • We prepared the stock solutions of FeSO4*7H2O ,KNO3, and KOH in the same method and at the same concentrations as prepared on February 4. The same hemoglobin stock solution as made yesterday was used.
  • We made the following ten ratios for the oven method. the same procedure as used on 1/29

Table 1: Preparation of Reaction Mixtures for Hemeglobin- Magnetite Nanoparticle/Fiber formation

Ratio concentration Hemoglobin (M) volume of Hemoglobin added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
10500 2.85714E-06 0.926058866 0.5 0.500624554 2.5 0.57331658
11000 2.72727E-06 0.883965281 0.5 0.500624554 2.5 0.615410165
11500 2.6087E-06 0.845532008 0.5 0.500624554 2.5 0.653843438
12000 0.0000025 0.810301508 0.5 0.500624554 2.5 0.689073939
12500 0.0000024 0.777889447 0.5 0.500624554 2.5 0.721485999
13000 2.30769E-06 0.747970622 0.5 0.500624554 2.5 0.751404824
13500 2.22222E-06 0.720268007 0.5 0.500624554 2.5 0.779107439
14000 2.14286E-06 0.694544149 0.5 0.500624554 2.5 0.804831297
14500 2.06897E-06 0.670594351 0.5 0.500624554 2.5 0.828781095
15000 0.000002 0.648241206 0.5 0.500624554 2.5 0.85113424


Data

Observations

  • For the oven prep reaction mixtures. It was noticed that immediately after adding the FeSO4*7H2O a dark(dark green/blue) precipitate in both small clusters and long string like shape formed in the Heme:Magnetite samples. We always add FeSO4*7H2O last into the solutions so that it does not prematurely react with the other compounds and to make sure it does not inhibit the reaction from proceeding.
  • Upon observing the BSA:Magnetite samples, it appeared that large clusters formed (leaf like structures) at the 12500:1 ratio and continued to form up to the 15000:1 ratio. Prior to the 12500:1 ratio, it appears a magnetic colloid suspension was formed, indicating that all ratios successfully formed magnetite nanoparticles. However, for potential fiber formation, it appears the 12500:1-15000:1 range has potential.