Difference between revisions of "User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==Day 5==
 
*  Goals
 
**UV-Vis the reaction mixtures prepared on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/05|2/5]]
 
**Prepare a new ratio of BSA- Magnetite solutions using both the oven method and the Schlenk line method with which to prepare them. This ratio will be the same as the hemoglobin- magnetite ratios prepared with the Schlenk line.
 
**Analyze the magnetite nanoparticles which were dried [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/05|Last week]] using FTIR
 
**Dry the particulate collected from the reaction mixtures prepared on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/05|2/5]] so that they may be used for FTIR and XRD next week.
 
 
 
==Procedure==
 
* We prepared the stock solutions of FeSO<sub>4</sub>*7H<sub>2</sub>O ,KNO<sub>3</sub>, and KOH in the same method and at the same concentrations as prepared on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04|February 4]]
 
* We made the following ten ratios for the oven method. the same procedure as used on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04|1/29]]
 
Table 1: Preparation of BSA and Magnetite solutions.
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 
| align="center" style="background:#f0f0f0;"|'''concentration BSA (M)'''
 
| align="center" style="background:#f0f0f0;"|'''volume of BSA added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
 
| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
 
|-
 
| 10500||2.85714E-06||0.568545766||0.5||0.500624554||2.5||0.93082968
 
|-
 
| 11000||2.72727E-06||0.542702776||0.5||0.500624554||2.5||0.95667267
 
|-
 
| 11500||2.6087E-06||0.519107003||0.5||0.500624554||2.5||0.980268443
 
|-
 
| 12000||0.0000025||0.497477545||0.5||0.500624554||2.5||1.001897901
 
|-
 
| 12500||0.0000024||0.477578443||0.5||0.500624554||2.5||1.021797003
 
|-
 
| 13000||2.30769E-06||0.459210041||0.5||0.500624554||2.5||1.040165405
 
|-
 
| 13500||2.22222E-06||0.442202262||0.5||0.500624554||2.5||1.057173184
 
|-
 
| 14000||2.14286E-06||0.426409324||0.5||0.500624554||2.5||1.072966122
 
|-
 
| 14500||2.06897E-06||0.411705554||0.5||0.500624554||2.5||1.087669892
 
|-
 
| 15000||0.000002||0.397982036||0.5||0.500624554||2.5||1.10139341
 
|}
 
 
 
*The second method we used was the Schlenk Line method- the same as was utilized on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29|1/29]]. 2 samples were made at the following concentrations
 
 
 
Table 2: Hemoglobin stock solution
 
 
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Preparation of hemoglobin Stock solution'''
 
| align="center" style="background:#f0f0f0;"|''''''
 
|-
 
| Molecular weight (g/mol)||64500
 
|-
 
| mass hemglobin (g)||0.0995
 
|-
 
| volume solution (L)||0.1
 
|-
 
| Concentration (M)||1.54264E-05
 
|}
 
 
 
Table 3: Hemoglobin- Magnetite solution ratios with Schlenk Method
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 
| align="center" style="background:#f0f0f0;"|'''concentration Hemoglobin (M)'''
 
| align="center" style="background:#f0f0f0;"|'''volume of Hemoglobin added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
 
| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
 
|-
 
| 19000||1.57895E-06||0.511769373||0.5||0.500624554||2.5||0.987606073
 
|-
 
| 21000||1.42857E-06||0.463029433||0.5||0.500624554||2.5||1.036346013
 
|}
 
 
 
'''The Drying of samples- General Protocol'''
 
#Make sure the test tube/ round bottom flask the securely closed and mix vigorously the prepared samples until all precipitate is suspended in the solution.
 
#Pour the newly mixed solutions into a clean beaker (about a 10mL one)
 
#Place a magnet at the bottom/ side of the vial and wait for all the precipitate to to collect on the glass, out of the solution.
 
#One the solution has cleared, pour the solution out into a falcon tube while still holding the magnet at the bottom of the beaker.
 
#Place beaker with precipitate into fume hood and allow to air dry at least 24 hours.
 
 
 
**To Run UV-Vis on the sample, do the following
 
#Centrifuge supernatant (collected solutions from above) for 30 minutes at 1500 rpm..
 
#Using ~1mL sample, analyze supernatant using UV/vis.
 
 
 
==Data==
 
For FTIR data and respective peaks please see [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/11| Moira's Notebook]]
 
 
 
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__NOTOC__
 
 
 
 
 
*UV-Vis
 
Corrected Absorbance Spectra of BSA:Magnetite samples with differing concentrations of KNO<sub>3</sub>
 
<br>
 
[[Image:MTP2112014.png]]
 
 
 
*There is an increased absorbance at ~300nm for the sample with a higher concentration of KNO<sub>3</sub>. This lead to the conclusion that the absorbance at ~300nm corresponds with nitrate. The lack of an iron absorbance peak indicates that the Fe was successfully incorporated into the magnetite nanoparticles or that a  protein fiber was created.
 

Latest revision as of 19:49, 19 April 2014

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