Difference between revisions of "User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/04"

From OpenWetWare
Jump to: navigation, search
(Entry title)
(Mb Mn-PPIX Purification)
Line 8: Line 8:
 
==Mb Mn-PPIX Purification==
 
==Mb Mn-PPIX Purification==
  
Dr. Hartings, Andrew, and I attempted to purify the Mn-Mb I made during the semester. We used the 100 mM Phosphate buffers, and everything eluted through the column. So, Andrew and I made 50 mM Tris buffer and 50 mM Tris/1M NaCl buffer. 6.057g of Tris was added to each, and 1 mol of NaCl (58.44g) were added, 900 mL of H<sub>2</sub>O were added, and titrated to pH 9 with 3M HCl. The remaining volume was added, each solution was filtered with a fritted funnel, and autoclaved.  
+
Dr. Hartings, Andrew, and I attempted to purify the Mn-Mb I made during the semester. We used the 100 mM Phosphate buffers, and everything eluted through the column. So, Andrew and I made 50 mM Tris buffer and 50 mM Tris/1M NaCl buffer. 6.057g of Tris was added to each, and 1 mol of NaCl (58.44g) were added, 900 mL of H<sub>2</sub>O were added, and titrated to pH 9 with 3M HCl. The remaining volume was added, each solution was filtered with a fritted funnel, and autoclaved. The purification will be run tomorrow.
  
  

Revision as of 10:41, 4 June 2014

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Mb Mn-PPIX Purification

Dr. Hartings, Andrew, and I attempted to purify the Mn-Mb I made during the semester. We used the 100 mM Phosphate buffers, and everything eluted through the column. So, Andrew and I made 50 mM Tris buffer and 50 mM Tris/1M NaCl buffer. 6.057g of Tris was added to each, and 1 mol of NaCl (58.44g) were added, 900 mL of H2O were added, and titrated to pH 9 with 3M HCl. The remaining volume was added, each solution was filtered with a fritted funnel, and autoclaved. The purification will be run tomorrow.