Difference between revisions of "User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/03"

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(Addition of Mn PPIX to Apo-Mb synthesized yesterday)
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==Addition of Mn PPIX to Apo-Mb synthesized yesterday==
 
==Addition of Mn PPIX to Apo-Mb synthesized yesterday==
  
Andrew and I are going to reconstitute the Mn-PPIX into the apo-Mb we prepared and left to run dialysis yesterday. For this, we are using the same protocol I ran a couple of months ago (link needs to be inserted).  
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Andrew and I are going to reconstitute the Mn-PPIX into the apo-Mb we prepared and left to run dialysis yesterday. For this, we are using the same protocol I ran a couple of months ago (link needs to be inserted). Assuming a complete conversion of the protein, we have 500 mg of apoprotein in solution. Since it's 2 mg PPIX per 10 mg of apoprotein, we will use 100 mg of PPIX.  
  
  

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Addition of Mn PPIX to Apo-Mb synthesized yesterday

Andrew and I are going to reconstitute the Mn-PPIX into the apo-Mb we prepared and left to run dialysis yesterday. For this, we are using the same protocol I ran a couple of months ago (link needs to be inserted). Assuming a complete conversion of the protein, we have 500 mg of apoprotein in solution. Since it's 2 mg PPIX per 10 mg of apoprotein, we will use 100 mg of PPIX.