Difference between revisions of "User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/02"

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(Preparation of Apo-Mb)
(Preparation of Apo-Mb)
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I am following the same procedure as [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2014/03/05| here] to prepare Apomyoglobin.
 
I am following the same procedure as [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2014/03/05| here] to prepare Apomyoglobin.
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The heme was extracted using the acid and 2-butanone method. Approximately 10 mL of the aqueous layer was produced. It was left to run dialysis overnight at 4°C in the 10 mM Na<sub>2</sub>CO<sub>3</sub> buffer.
  
 
==Synthesis of Cobalt PPIX==
 
==Synthesis of Cobalt PPIX==

Revision as of 10:58, 2 June 2014

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Preparation of Apo-Mb

I am following the same procedure as here to prepare Apomyoglobin.

The heme was extracted using the acid and 2-butanone method. Approximately 10 mL of the aqueous layer was produced. It was left to run dialysis overnight at 4°C in the 10 mM Na2CO3 buffer.

Synthesis of Cobalt PPIX