Difference between revisions of "User:Paul Rothenberg/Notebook/Pauls Notebook/2014/03/26"

From OpenWetWare
Jump to: navigation, search
(Entry title)
(UV-VIS of Apoprotein)
Line 7: Line 7:
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
==UV-VIS of Apoprotein==
 
==UV-VIS of Apoprotein==
* UV-VIS spectroscopy was run on the protein to determine if the extraction of the heme groups was successful. If so, there should be a peak at 280 nm. If there is heme left, there would be a peak at 400 nm.  
+
* UV-VIS spectroscopy was run on the protein to determine if the extraction of the heme groups was successful. If so, there should be a peak at 280 nm. If there is heme left, there would be a peak at 400 nm.
  
 +
*The spectrum revealed a large peak at 280 nm (roughly 0.7) and a very slight peak at 400 (0.06-0.07).
  
 
==Reconstitution of Apoprotein==
 
==Reconstitution of Apoprotein==

Revision as of 07:23, 26 March 2014

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

UV-VIS of Apoprotein

  • UV-VIS spectroscopy was run on the protein to determine if the extraction of the heme groups was successful. If so, there should be a peak at 280 nm. If there is heme left, there would be a peak at 400 nm.
  • The spectrum revealed a large peak at 280 nm (roughly 0.7) and a very slight peak at 400 (0.06-0.07).

Reconstitution of Apoprotein