Difference between revisions of "User:Paul Rothenberg/Notebook/Pauls Notebook/2014/03/05"

From OpenWetWare
Jump to: navigation, search
(Heme Extraction from Myoglobin)
(Heme Extraction from Myoglobin)
Line 10: Line 10:
 
#24.3 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 200 mg of myoglobin
 
#24.3 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 200 mg of myoglobin
 
# 3.36 g of Sodium Bicarbonate was dissolved in 4L of H<sub>2</sub>O to make a 10 mM dialysis buffer
 
# 3.36 g of Sodium Bicarbonate was dissolved in 4L of H<sub>2</sub>O to make a 10 mM dialysis buffer
#
+
#The pH of the Tris solution was brought down to 2.3-2.5 at 0°C before 2-butanone extraction was performed.
  
  

Revision as of 08:27, 5 March 2014

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Heme Extraction from Myoglobin

  • Preparation of Apoprotein:
  1. 24.3 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 200 mg of myoglobin
  2. 3.36 g of Sodium Bicarbonate was dissolved in 4L of H2O to make a 10 mM dialysis buffer
  3. The pH of the Tris solution was brought down to 2.3-2.5 at 0°C before 2-butanone extraction was performed.