Difference between revisions of "User:Paul Rothenberg/Notebook/Pauls Notebook/2014/03/05"

From OpenWetWare
Jump to: navigation, search
(Heme Extraction from Myoglobin)
(Heme Extraction from Myoglobin)
Line 8: Line 8:
 
==Heme Extraction from Myoglobin==
 
==Heme Extraction from Myoglobin==
 
* Preparation of Apoprotein:
 
* Preparation of Apoprotein:
#24.228 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 1 gram of myoglobin
+
#24.3 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 200 mg of myoglobin
 +
# 3.36 g of Sodium Bicarbonate was dissolved in 4L of H<sub>2</sub>O to make a 10 mM dialysis buffer
 +
#
  
  

Revision as of 07:19, 5 March 2014

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Heme Extraction from Myoglobin

  • Preparation of Apoprotein:
  1. 24.3 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 200 mg of myoglobin
  2. 3.36 g of Sodium Bicarbonate was dissolved in 4L of H2O to make a 10 mM dialysis buffer