Difference between revisions of "User:Paul Rothenberg/Notebook/Pauls Notebook/2013/07/01"

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(Synthesis of Buffered Mb)
(Reaction of OPD+H2O2 with Mb/Acetate Catalyst in H2O)
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<b>Trial 2 Data:</b>
 
<b>Trial 2 Data:</b>
  
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[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_GRAPH_Trial2.png]]
  
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[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_SEQUENTIAL_GRAPH_Trial2.png]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_GRAPH_Trial2.png]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_SEQUENTIAL_GRAPH_Trial2.png]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_GRAPH_Trial2.png]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_SEQUENTIAL_GRAPH_Trial2.png]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_GRAPH_Trial2.png]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_SEQUENTIAL_GRAPH_Trial2.png]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_GRAPH_Trial2.png]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_SEQUENTIAL_GRAPH_Trial2.png]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_GRAPH_Trial2.png]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_SEQUENTIAL_GRAPH_Trial2.png]]
 
<b>Trial 3 Data:</b>
 
<b>Trial 3 Data:</b>
  

Revision as of 08:59, 1 July 2013

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Reaction of OPD+H2O2 with Mb/Acetate Catalyst in H2O

Going to continue the trials in H2O with Mb/Acetate. We are also going to make up more buffered protein solutions.

Trial 2 Data:

Mb Acetate OPD H2O2 H2O 5C GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 5C SEQUENTIAL GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 15C GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 15C SEQUENTIAL GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 25C GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 25C SEQUENTIAL GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 35C GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 35C SEQUENTIAL GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 45C GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 45C SEQUENTIAL GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 55C GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 55C SEQUENTIAL GRAPH Trial2.png Trial 3 Data:

Synthesis of Buffered Mb

This synthesis was taken from May 22.

5 mg of Mb and approximately 490-500 milligrams of KCl were added to four tubes, and 20 mL of pH 3 Citrate, pH 5 Acetate, pH 7 Phosphate, and pH 9 Tris buffers were added.