Difference between revisions of "User:Paul Rothenberg/Notebook/Pauls Notebook/2013/07/01"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Entry title==
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==Reaction of OPD+H<sub>2</sub>O<sub>2</sub> with Mb/Acetate Catalyst in H<sub>2</sub>O==
* Insert content here...
 
  
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Going to continue the trials in H<sub>2</sub>O with Mb/Acetate. We are also going to make up more buffered protein solutions.
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<b>Trial 2 Data:</b>
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[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_GRAPH_Trial2.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_SEQUENTIAL_GRAPH_Trial2.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_GRAPH_Trial2.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_SEQUENTIAL_GRAPH_Trial2.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_GRAPH_Trial2.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_SEQUENTIAL_GRAPH_Trial2.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_GRAPH_Trial2.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_SEQUENTIAL_GRAPH_Trial2.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_GRAPH_Trial2.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_SEQUENTIAL_GRAPH_Trial2.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_GRAPH_Trial2.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_SEQUENTIAL_GRAPH_Trial2.png|750px]]
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<b>Trial 3 Data:</b>
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[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_65C_GRAPH_Trial3.png|750px]]
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[[Image:Mb_Acetate_OPD_H2O2_H2O_65C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
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== Synthesis of Buffered Mb ==
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This synthesis was taken from [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/22 May 22].
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5 mg of Mb and approximately 490-500 milligrams of KCl were added to four tubes, and 20 mL of pH 3 Citrate, pH 5 Acetate, pH 7 Phosphate, and pH 9 Tris buffers were added.
  
 
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Latest revision as of 22:53, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Reaction of OPD+H2O2 with Mb/Acetate Catalyst in H2O

Going to continue the trials in H2O with Mb/Acetate. We are also going to make up more buffered protein solutions.

Trial 2 Data:

Mb Acetate OPD H2O2 H2O 5C GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 5C SEQUENTIAL GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 15C GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 15C SEQUENTIAL GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 25C GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 25C SEQUENTIAL GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 35C GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 35C SEQUENTIAL GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 45C GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 45C SEQUENTIAL GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 55C GRAPH Trial2.png

Mb Acetate OPD H2O2 H2O 55C SEQUENTIAL GRAPH Trial2.png

Trial 3 Data:

Mb Acetate OPD H2O2 H2O 5C GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 5C SEQUENTIAL GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 15C GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 15C SEQUENTIAL GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 25C GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 25C SEQUENTIAL GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 35C GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 35C SEQUENTIAL GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 45C GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 45C SEQUENTIAL GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 55C GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 55C SEQUENTIAL GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 65C GRAPH Trial3.png

Mb Acetate OPD H2O2 H2O 65C SEQUENTIAL GRAPH Trial3.png

Synthesis of Buffered Mb

This synthesis was taken from May 22.

5 mg of Mb and approximately 490-500 milligrams of KCl were added to four tubes, and 20 mL of pH 3 Citrate, pH 5 Acetate, pH 7 Phosphate, and pH 9 Tris buffers were added.