Difference between revisions of "User:Paul Rothenberg/Notebook/Pauls Notebook/2013/06/05"

From OpenWetWare
Jump to: navigation, search
m
(fix raw html notebook nav)
 
(5 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
|-
 
|-
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
==Reaction of OPD+H<sub>2</sub>O<sub>2</sub> with Mb/Citrate Catalyst in DMSO==
 
==Reaction of OPD+H<sub>2</sub>O<sub>2</sub> with Mb/Citrate Catalyst in DMSO==
* Today, we are going back to running this reaction by dissolving the reactants, not suspending them. The same procedure will be followed as in previous reactions, just with DMSO as a solvent.  
+
Today, we are going back to running this reaction by dissolving the reactants, not suspending them. The same procedure will be followed as in previous reactions, just with DMSO as a solvent.  
  
 
<b>Data:</b>
 
<b>Data:</b>
 +
 +
 
[[Image:Mb_Citrate_OPD_H2O2_DMSO_5C_WORKUP_GRAPH.png|750px]]
 
[[Image:Mb_Citrate_OPD_H2O2_DMSO_5C_WORKUP_GRAPH.png|750px]]
  
 
The DMSO froze at 5°C. The graph indicated no absorbance at the final time point.  
 
The DMSO froze at 5°C. The graph indicated no absorbance at the final time point.  
  
[[Image:Mb_Citrate_OPD_H2O2_DMSO_15C_WORKUP_GRAPH.png|750px]]
+
[[Image:Mb_Citrate_OPD_H2O2_DMSO_15C_WORKUP_GRAPH.png|375px]] [[Image:Mb_Citrate_OPD_H2O2_DMSO_15C_SEQUENTIAL_WORKUP_GRAPH.png|375px]] <br>
 
 
[[Image:Mb_Citrate_OPD_H2O2_DMSO_15C_SEQUENTIAL_WORKUP_GRAPH.png|750px]]
 
 
 
[[Image:Mb_Citrate_OPD_H2O2_DMSO_25C_WORKUP_GRAPH.png|750px]]
 
  
[[Image:Mb_Citrate_OPD_H2O2_DMSO_25C_SEQUENTIAL_WORKUP_GRAPH.png|750px]]
+
[[Image:Mb_Citrate_OPD_H2O2_DMSO_25C_WORKUP_GRAPH.png|375px]] [[Image:Mb_Citrate_OPD_H2O2_DMSO_25C_SEQUENTIAL_WORKUP_GRAPH.png|375px]] <br>
  
[[Image:Mb_Citrate_OPD_H2O2_DMSO_35C_WORKUP_GRAPH.png|750px]]
+
[[Image:Mb_Citrate_OPD_H2O2_DMSO_35C_WORKUP_GRAPH.png|375px]] [[Image:Mb_Citrate_OPD_H2O2_DMSO_35C_SEQUENTIAL_WORKUP_GRAPH.png|375px]] <br>
 
 
[[Image:Mb_Citrate_OPD_H2O2_DMSO_35C_SEQUENTIAL_WORKUP_GRAPH.png|750px]]
 
 
 
[[Image:Mb_Citrate_OPD_H2O2_DMSO_45C_WORKUP_GRAPH.png|750px]]
 
 
 
[[Image:Mb_Citrate_OPD_H2O2_DMSO_45C_SEQUENTIAL_WORKUP_GRAPH.png|750px]]
 
 
 
All of the reactions did not have as high of absorbances as Mb/Citrate in other solvents. There was also no defined peaks at 420 or 460 nm, as seen previously. Instead, the initial peak is at 400 nm, and that declines over the time interval. The curves are also not smooth, as compared to previous reactions. The same dip in the graph, seen just over 650 nm, was also seen yesterday when running the same reaction in suspension.
 
  
 +
[[Image:Mb_Citrate_OPD_H2O2_DMSO_45C_WORKUP_GRAPH.png|375px]] [[Image:Mb_Citrate_OPD_H2O2_DMSO_45C_SEQUENTIAL_WORKUP_GRAPH.png|375px]] <br>
  
 +
All of the reactions did not have as high of absorbances as Mb/Citrate in other solvents. There was also no defined peaks at 420 or 460 nm, as seen previously. Instead, the initial peak is at 400 nm, and that declines over the time interval. The curves are also not smooth, as compared to previous reactions. The same dip in the graph, seen just over 650 nm, was also seen yesterday when running the same reaction in suspension.
  
 
== Reaction of OPD+H<sub>2</sub>O<sub>2</sub> with Mb/Phosphate Catalyst in DMSO ==
 
== Reaction of OPD+H<sub>2</sub>O<sub>2</sub> with Mb/Phosphate Catalyst in DMSO ==
Line 40: Line 32:
 
<b>Data:</b>
 
<b>Data:</b>
  
[[Image:Mb_Phosphate_OPD_H2O2_DMSO_25C_WORKUP_GRAPH.png|750px]]
+
[[Image:Mb_Phosphate_OPD_H2O2_DMSO_25C_WORKUP_GRAPH.png|375px]] [[Image:Mb_Phosphate_OPD_H2O2_DMSO_25C_SEQUENTIAL_WORKUP_GRAPH.png|375px]]
 
 
[[Image:Mb_Phosphate_OPD_H2O2_DMSO_25C_SEQUENTIAL_WORKUP_GRAPH.png|750px]]
 
 
 
[[Image:Mb_Phosphate_OPD_H2O2_DMSO_35C_WORKUP_GRAPH.png|750px]]
 
 
 
[[Image:Mb_Phosphate_OPD_H2O2_DMSO_35C_SEQUENTIAL_WORKUP_GRAPH.png|750px]]
 
  
[[Image:Mb_Phosphate_OPD_H2O2_DMSO_45C_WORKUP_GRAPH.png|750px]]
+
[[Image:Mb_Phosphate_OPD_H2O2_DMSO_35C_WORKUP_GRAPH.png|375px]] [[Image:Mb_Phosphate_OPD_H2O2_DMSO_35C_SEQUENTIAL_WORKUP_GRAPH.png|375px]]
  
[[Image:Mb_Phosphate_OPD_H2O2_DMSO_45C_SEQUENTIAL_WORKUP_GRAPH.png|750px]]
+
[[Image:Mb_Phosphate_OPD_H2O2_DMSO_45C_WORKUP_GRAPH.png|375px]] [[Image:Mb_Phosphate_OPD_H2O2_DMSO_45C_SEQUENTIAL_WORKUP_GRAPH.png|375px]]
  
  

Latest revision as of 21:44, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Reaction of OPD+H2O2 with Mb/Citrate Catalyst in DMSO

Today, we are going back to running this reaction by dissolving the reactants, not suspending them. The same procedure will be followed as in previous reactions, just with DMSO as a solvent.

Data:


Mb Citrate OPD H2O2 DMSO 5C WORKUP GRAPH.png

The DMSO froze at 5°C. The graph indicated no absorbance at the final time point.

Mb Citrate OPD H2O2 DMSO 15C WORKUP GRAPH.png Mb Citrate OPD H2O2 DMSO 15C SEQUENTIAL WORKUP GRAPH.png

Mb Citrate OPD H2O2 DMSO 25C WORKUP GRAPH.png Mb Citrate OPD H2O2 DMSO 25C SEQUENTIAL WORKUP GRAPH.png

Mb Citrate OPD H2O2 DMSO 35C WORKUP GRAPH.png Mb Citrate OPD H2O2 DMSO 35C SEQUENTIAL WORKUP GRAPH.png

Mb Citrate OPD H2O2 DMSO 45C WORKUP GRAPH.png Mb Citrate OPD H2O2 DMSO 45C SEQUENTIAL WORKUP GRAPH.png

All of the reactions did not have as high of absorbances as Mb/Citrate in other solvents. There was also no defined peaks at 420 or 460 nm, as seen previously. Instead, the initial peak is at 400 nm, and that declines over the time interval. The curves are also not smooth, as compared to previous reactions. The same dip in the graph, seen just over 650 nm, was also seen yesterday when running the same reaction in suspension.

Reaction of OPD+H2O2 with Mb/Phosphate Catalyst in DMSO

Due to the DMSO freezing at 5°C, the reaction was only run at 25°C, 35°C, and 45°C.

Data:

Mb Phosphate OPD H2O2 DMSO 25C WORKUP GRAPH.png Mb Phosphate OPD H2O2 DMSO 25C SEQUENTIAL WORKUP GRAPH.png

Mb Phosphate OPD H2O2 DMSO 35C WORKUP GRAPH.png Mb Phosphate OPD H2O2 DMSO 35C SEQUENTIAL WORKUP GRAPH.png

Mb Phosphate OPD H2O2 DMSO 45C WORKUP GRAPH.png Mb Phosphate OPD H2O2 DMSO 45C SEQUENTIAL WORKUP GRAPH.png