Difference between revisions of "User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/20"

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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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Latest revision as of 21:42, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Reaction of OPD and H2O2 in MeOH with Mb/Citrate Catalyst

  • Objectives:
  • 1. Analyze the reaction of O-phenylenediamine and hydrogen peroxide catalyzed by Mb in KCl/Citrate



  • 1. Added 18 mg of O-phenylenediamine to 18 mL of MeOH and vortexed
  • 2. Added 15 mg of Mb lyopholized in KCl/Citrate to 1.5 mL MeOH and vortexed
  • 3. Added 1.134 mL of 30% hydrogen peroxide to 8.866 mL MeOH
  • 4. Centrifuged the Mb solution at 13,200 rpm for 10 minutes
  • 5. The spectrum was taken at 5C, and the H2O2 solution was added at 1% spectrum completion
  • 6. Spectra were then taken at 15C, 25C, 35C, and 45C
  • 7. The data was then analyzed and the spectra were converted to absorbance and corrected
  • Graphs:

Mb Citrate 5C WORKUP GRAPH CORRECTED.png Mb Citrate 5C SEQUENTIAL WORKUP GRAPH CORRECTED.png

Mb Citrate 15C WORKUP GRAPH CORRECTED.png Mb Citrate 15C SEQUENTIAL WORKUP GRAPH CORRECTED.png

Mb Citrate 25C WORKUP GRAPH CORRECTED.png Mb Citrate 25C SEQUENTIAL WORKUP GRAPH CORRECTED.png

Mb Citrate 35C WORKUP GRAPH CORRECTED.png Mb Citrate 35C SEQUENTIAL WORKUP GRAPH CORRECTED.png

Mb Citrate 45C WORKUP GRAPH CORRECTED.png Mb Citrate 45C SEQUENTIAL WORKUP GRAPH CORRECTED.png



  • Observations:
  • 1. Condensation during the 5 Centigrade spectrum - non-useful data
  • 2. The baseline was much higher during the 15C spectrum - will revisit during data analysis
  • 3. Condensation on the 25C spectrum - take note
  • 4. The spectra had different behaviors and peaks between 300 and 800 nm - need to determine what is being synthesized at these peaks