User:Paul Rothenberg/Notebook/Pauls Notebook/2013/03/04: Difference between revisions
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== | ==4 March 2013== | ||
* | * Objectives: | ||
*1. Synthesize two buffers to purify Nickel Hemoglobin previously synthesized | |||
*Procedure: | |||
*1. 10 mM Tris (1.21g) and 50 mM NaCl (2.922g) were added, along with 900 mL of distilled water | |||
*2. The pH was brought to 9.5 using 1M NaOH and 3 M HCl, and the remaining 100 mL of distilled water were added | |||
*3. 10 mM Tris (1.21g) and 1M NaCl (58.44g) were added with 900 mL of distilled water | |||
*4. The pH was brought to 9.5 using 1M NaOH, and the remaining 100 mL of distilled water were added | |||
*5. 10 mM Tris and 50 mM NaCl were added along with 3,975 mL of distilled water | |||
*6. The pH was brought to 9.5 using 3M HCl, and the remaining 25 mL of water were added | |||
*7. The resulting buffer was used as a dialysis solution, exchanging previously synthesized Ni Hb in pH 8.5 solution | |||
*8. The Ni Hb was placed in two dialysis bags and placed in the pH 9.5 solution in a cold environment, left overnight | |||
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